Method and system for the simultaneous and multiple detection and quantification of the hybridization of molecular compounds such as nucleic acids, dna rna, pna, and proteins
OwnerMario Raimondo Cariainventor
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Abstract
The method is used for detecting a position of several hybridization sites on a support (2001) containing probes (3002) having hybridized targets (3003) remaining attached thereto after a washing step. It comprises the steps of: emitting a radiation (3004) from a source (3001) towards the support (2001); receiving the radiation coming from the support on a microelectrode detector (1002) sensitive to the radiation; and quantifying the targets in different sites of the support at the same time.
Claims44
A method for detecting a position of several hybridization sites on a support (2001) containing probes (3002;
- 4002) possibly having hybridized targets (3003;
- 4003) remaining attached thereto after a washing step, characterized in that it comprises the steps of:
- emitting a radiation (3004;
- 4004) from a source (3001;
- 4001) towards the support (2001);
- receiving a radiation coming from the support on a microelectrode detector (1002) sensitive to the radiation;
- and
- quantifying different sites of the support at the same time concerning possible hybridized targets.
The method of any of claims.1 to 9, characterized in that the radiation is a laser beam.
A device for detecting a position of several hybridization sites on a support (2001) containing deposited probes (3002;
- 4002) possibly having hybridized targets (3003;
- 4003) remaining attached thereto after a washing step, characterized in that it comprises:
- a support (2001) for the probes;
- a source (3001;
- 4001) for emitting a radiation (3004;
- 4004) towards the support;
- a microelectrode detector (1002) arranged to receive a radiation coming from the support and sensitive thereto;
- and
- means (3006, 3007;
- 4006, 4007) for quantifying different sites of the support at the same time concerning possible hybridized targets.
Dependencies are inferred from the claim text.