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US 2003/0143573 A1Published application
Detection of bovine viral diarrhea virus in tissue samples
AssigneesCornell Research Foundation, Inc., IDEXX Laboratories, Inc.
InventorsRoy Huchzermeier, Edward Joseph Dubovi
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Abstract
The present invention relates to a method of detecting whether a target animal is Bovine Viral Diarrhea Virus (BVDV) positive or negative by determining whether a gp48 protein-specific reagent binds to a gp48 Bovine Viral Diarrhea Virus protein or protein fragment, which retains antigenic specificity, from a target animal's tissue sample.
Claims24
A method of detecting whether a target animal is Bovine Viral Diarrhea Virus positive or negative comprising:
- a) providing a tissue sample from said target animal;
- b) providing an assay system which comprises:
- 1) a capture antibody that is a Bovine Viral Diarrhea Virus epitope specific antibody, said capture antibody being immobilized on a solid support and capable of recognizing and binding a gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof which retains antigenic specificity;
- 2) a detector antibody that is an anti-Bovine Viral Diarrhea Virus antibody, capable of recognizing and binding said gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof which retains antigenic specificity;
- and
- 3) a signal generator for indicating the presence of said detector antibody operatively associated with Bovine Viral Diarrhea Virus antigen;
- c) analyzing the sample with said assay system to generate a change in signal if Bovine Viral Diarrhea Virus antigen is present in the sample;
- and
- d) comparing the signal to one or more reference levels to indicate whether the target animal is Bovine Viral Diarrhea Virus positive or negative.
6 more elements
A method of detecting Bovine Viral Diarrhea Virus infection in a bovine comprising:
- providing a tissue sample from the bovine;
- contacting the sample with a gp48 Bovine Viral Diarrhea Virus protein-specific reagent;
- and
- analyzing whether the gp48 Bovine Viral Diarrhea Virus protein-specific reagent binds to a gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof, which retains antigenic specificity, from the sample.
1 more element
Dependencies are inferred from the claim text.