Molecular diagnostic and computerized decision support system for selecting the optimum treatment for human cancer

A method for selecting the optimum treatment for a cancerous condition in a human patient, the method comprising:

• preparing PCR kit and gene chip;
• isolating mRNA from a patient's tumor or blood sample with an extraction buffer;
• synthesizing and amplifying cDNA in a patient's tumor or blood sample with primers highly specific for targeted cancer genes;
• detecting cancer genes, mutations using a gene chip;
• analyzing and interpreting PCR and/or gene chip results using a detector linked to a computerized decisions support system running a diagnostic software program with accompanying database for providing an indication of the drug which is optimum for treating the patient's cancer with the least likely chance for a drug side effect.

A method using a clinical computerized decision support system for selecting the optimum treatment for a patient suffering from breast cancer, the method comprising:

• combining a gene chip with PCR primers for the detection of particular breast cancer genes in a sample of the patient's tissue or blood;
• optically inspecting the resulting chemical and biological reaction using a automated detector;
• correlating using the software output from the integrated detector with a disease analytical models database and/or a database comprising the results of clinical studies testing the efficacy and toxicity of various drugs in treating patients with particular genotypes having breast cancer;
• and
• providing an indication of one or more drugs which is optimum for treating the patient's breast cancer with the most effective outcome and the least amount of side effect.