Infectious disease microarray
AssigneeNorth Carolina State University
InventorsNicholas J.H. Sharp, Scott J. Schatzberg, Gregory Robert OBrian
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Abstract
A method for detecting one or more pathogens in a subject. The method includes the steps of: (a) procuring a biological sample, wherein the biological sample comprises nucleic acid material; (b) amplifying the nucleic acid material using random primers to produce a set of random amplicons; (c) providing one or more pathogen-specific probes or probe sets; (d) hybridizing the set of random amplicons with the one or more pathogen-specific probes or probe sets; and (e) determining selective hybridization between a random amplicon and a pathogen-specific probe or probe set, whereby the presence of a pathogen in a biological sample is detected.
Claims51
A method for detecting a pathogen in a biological sample, the method comprising:
- (a) procuring a biological sample, wherein the biological sample comprises nucleic acid material;
- (b) amplifying the nucleic acid material using random primers to produce a set of random amplicons;
- (c) providing one or more pathogen-specific probes;
- (d) hybridizing the set of random amplicons with the one or more pathogen-specific probes;
- and
- (e) determining selective hybridization between a random amplicon and a pathogen-specific probe, whereby the presence of a pathogen in a subject is detected.
A method for detecting a pathogen in a biological sample, the method comprising:
- (a) procuring a biological sample, wherein the biological sample comprises nucleic acid material;
- (b) amplifying the nucleic acid material using random primers to produce a set of random amplicons;
- (c) providing one or more pathogen-specific probe sets;
- (d) hybridizing the set of random amplicons with the one or more pathogen-specific probe sets;
- and
- (e) determining selective hybridization between a random amplicon and a pathogen-specific probe set, whereby the presence of a pathogen in a biological sample is detected.
A microarray for detecting a pathogen in a biological sample comprising:
- (a) a solid support comprising a plurality of identifying positions;
- and
- (b) one or more pathogen-specific probe sets, each probe set occupying one of the plurality of identifying positions on the solid support.
Dependencies are inferred from the claim text.