Method for labeling and fragmenting DNA
AssigneeBio Merieux
InventorsCecile Bourget, Mitsuharu Kotera, Jean Lhomme, Emmanuelle Trevisiol, Ali Laayoun, Christelle Tora, Isabelle Sothier
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Abstract
The invention relates to a method for labeling and fragmenting a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by creating at least one abasic site on said DNA, attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment. The invention finds a preferred application in the field of diagnosis.
Claims28
A method for labeling and fragmenting a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:
- fragmenting the DNA by creating at least one abasic site on said DNA,
- attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment.
Dependencies are inferred from the claim text.