Method for DNA sequencing and gene identification
AssigneeEastman Kodak Company
InventorsZhihao Yang, Tiecheng A. Qiao
Life of this patent
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Abstract
A method for single molecule identification of a target DNA molecule in a random coil state having the following steps: a) attaching an optically distinguishable material to a DNA sequence recognition unit; b) hybridizing the DNA sequence recognition unit to the target DNA molecule in a random coil state to form a hybridized DNA complex in a random coil state; c) stretching the hybridized DNA complex in a random coil state to form a hybridized DNA complex in a substantially linear configuration; and d) detecting the optically distinguishable material in a sequential manner along the substantially linear hybridized DNA complex, thereby identifying the target DNA molecule.
Claims14
A method for single molecule identification of a target DNA molecule in a random coil state comprising the following steps:
- a) attaching an optically distinguishable material to a DNA sequence recognition unit;
- b) hybridizing said DNA sequence recognition unit to said target DNA molecule in a random coil state to form a hybridized DNA complex in a random coil state;
- c) stretching said hybridized DNA complex in a random coil state to form a hybridized DNA complex in a substantially linear configuration;
- and
- d) detecting said optically distinguishable material in a sequential manner along said substantially linear hybridized DNA complex, thereby identifying said target DNA molecule.
A method for single molecule identification of a target DNA molecule in a random coil state comprising the following steps:
- a) stretching said target DNA molecule in a random coil state to form a substantially linear configuration;
- b) attaching an optically distinguishable material to a DNA sequence recognition unit;
- c) hybridizing said DNA sequence recognition unit to said target DNA molecule in a substantially linear configuration to form a hybridized DNA complex in a substantially linear configuration;
- and
- d) detecting said optically distinguishable material in a sequential manner along said substantially linear hybridized DNA complex, thereby identifying said target DNA molecule.
Dependencies are inferred from the claim text.