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US 2003/0143532 A1Published application
Method for producing biochemical analysis data and apparatus used therefor
AssigneeFuji Photo Film Co., Ltd.
InventorNobuhiko Ogura
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AssigneeFuji Photo Film Co., Ltd.
InventorNobuhiko Ogura
Estimating the term…
A method for producing biochemical analysis data includes the steps of selectively binding a substance derived from a living organism and labeled with a labeling substance which generates chemiluminescence emission when it contacts a chemiluminescent substrate with specific binding substances contained in a plurality of absorptive regions formed in a biochemical analysis unit, bringing the labeling substance into contact with a chemiluminescent substrate, thereby causing it to release chemiluminescence emission, producing a first digital signal for each of the plurality of absorptive regions of the biochemical analysis unit based on an analog data obtained by detecting the chemiluminescence emission for a first exposure time using a solid state area sensor, storing the first digital signals in a memory, producing a second digital signal for each of the plurality of absorptive regions of the biochemical analysis unit based on an analog data obtained by detecting the chemiluminescence emission for a second exposure time, storing the second digital signal in the memory, comparing a signal intensity of the second digital signal with a saturated value of the signal intensity, determining a signal intensity of a second digital signal which is lower than the saturated value as biochemical analysis data of the corresponding absorptive region of the biochemical analysis unit to store it in the memory, and adopting a signal intensity of a first digital signal when a signal intensity of a second digital signal is equal to or higher than the saturated value, thereby producing biochemical analysis data.
A method for producing biochemical analysis data comprising steps of selectively binding a substance derived from a living organism and labeled with a labeling substance which generates chemiluminescence emission when it contacts a chemiluminescent substrate with specific binding substances whose structure and characteristics are known and which are contained in a plurality of absorptive regions formed in a biochemical analysis unit so as to be spaced apart from each other, or selectively binding a substance derived from a living organism and labeled with a hapten with the specific binding substances whose structure and characteristics are known and which are contained in the plurality of absorptive regions formed in the biochemical analysis unit so as to be spaced apart from each other and binding an antibody for the hapten labeled with an enzyme having a property to generate chemiluminescence emission when it contacts a chemiluminescent substrate with the hapten by an antigen-antibody reaction, bringing the labeling substance selectively contained in the plurality of absorptive regions of the biochemical analysis unit into contact with a chemiluminescent substrate, thereby causing it to release chemiluminescence emission, photoelectrically detecting the chemiluminescence emission released from the plurality of absorptive regions of the biochemical analysis unit for a first exposure time period using a solid state area sensor to produce an analog signal for each of the plurality of absorptive regions of the biochemical analysis unit, digitizing the analog signal to produce a digital signal for each of the plurality of absorptive regions of the biochemical analysis unit, storing the digital signals in a memory, photoelectrically detecting the chemiluminescence emission released from the plurality of absorptive regions of the biochemical analysis unit for a second exposure time period longer than the first exposure time period to produce an analog signal for each of the plurality of absorptive regions of the biochemical analysis unit, digitizing the analog signal to produce a digital signal for each of the plurality of absorptive regions of the biochemical analysis unit, storing the digital signals in the memory, comparing a signal intensity of the digital signal produced by photoelectrically detecting the chemiluminescence emission released from each of the absorptive regions of the biochemical analysis unit for the second exposure time period and stored in the memory with a saturated value of the signal intensity determining a signal intensity of a digital signal which is lower than the saturated value as biochemical analysis data of the corresponding absorptive region of the biochemical analysis unit to store it in the memory, and adopting a signal intensity of a digital signal which is generated by photoelectrically detecting the chemiluminescence emission released from an absorptive region of the biochemical analysis unit for the first exposure time period and stored in the memory when a signal intensity of a digital signal generated by photoelectrically detecting the chemiluminescence emission released from the absorptive region of the biochemical analysis unit for the second exposure time period is equal to or higher than the saturated value, thereby producing biochemical analysis data.
An apparatus for producing biochemical analysis data comprising a sample stage for placing a biochemical analysis unit in which a plurality of absorptive regions are formed so as to be spaced apart from each other and a substance derived from a living organism and labeled with a labeling substance which generates chemiluminescence emission when it contacts a chemiluminescent substrate is selectively bound with specific binding substances whose structure or characteristics are known contained in the plurality of absorptive regions, a solid state area sensor for photoelectrically detecting chemiluminescence emission released from the plurality of absorptive regions of the biochemical analysis unit and generating an analog signal for each of the plurality of absorptive regions of the biochemical analysis unit, a plurality of light guide members disposed in such a manner that each of light collecting end portions of the light guide members faces one of the plurality of absorptive regions of the biochemical analysis unit placed on the sample stage and adapted for leading chemiluminescence emission released from the plurality of absorptive regions of the biochemical analysis unit to the solid state area sensor, an A/D converter for digitizing analog signals generated by the solid state area sensor to produce a digital signal for each of the plurality of absorptive regions of the biochemical analysis unit, a memory for storing the digital signal generated by the A/D converter for each of the plurality of absorptive regions of the biochemical analysis unit, data processing means for producing biochemical analysis data for each of the plurality of absorptive regions of the biochemical analysis unit based on the digital signal of each of the plurality of absorptive regions of the biochemical analysis unit, and a control means for controlling the solid state area sensor, the A/D converter and the data processing means, the control means being adapted for controlling the solid state area sensor so as to photoelectrically detect chemiluminescence emission released from the absorptive regions of the biochemical analysis unit for a first exposure time period, thereby generating an analog signal for each of the absorptive regions of the biochemical analysis unit and to photoelectrically detect chemiluminescence emission released from the absorptive regions of the biochemical analysis unit for a second exposure time period longer than the first exposure time period, thereby generating an analog signal for each of the absorptive regions of the biochemical analysis unit, controlling the A/D converter so as to digitize the analog signal of each of the absorptive regions of the biochemical analysis unit generated by the solid state area sensor and to store it in the memory, and controlling the data processing means to compare a signal intensity of the digital signal of each of the absorptive regions of the biochemical analysis unit generated by photoelectrically detecting chemiluminescence emission released from the absorptive regions of the biochemical analysis unit for the second exposure time period and stored in the memory with a saturated value of the signal intensity, to determine the signal intensity of the digital signal which is lower than the saturated value as biochemical analysis data of the absorptive region of the biochemical analysis unit, to store them in the memory, and to adopt a signal intensity of a digital signal which is generated by photoelectrically detecting the chemiluminescence emission released from an absorptive region of the biochemical analysis unit for the first exposure time period and stored in the memory when a signal intensity of a digital signal generated by photoelectrically detecting the chemiluminescence emission released from the absorptive region of the biochemical analysis unit for the second exposure time period is equal to or higher than the saturated value, thereby producing biochemical analysis data of the plurality of the biochemical analysis unit.
Dependencies are inferred from the claim text.