Method and system for determining the amount, distribution and conformation of genetic material in a cell
OwnerBoaz Lernerinventor
Life of this patent
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Abstract
A method and system for genetic analysis of cells or nuclei. For each of one or more DNA sequences in the cells or nuclei, the DNA sequence is labeled with a probe producing a detectable optical signal. A two-dimensional image of the cell or nucleus is obtained that is focused in each of a plurality of parallel planes passing through the cell or nucleus. The number of optical signals from the DNA sequence in each image having one or more predetermined characteristics is determined, and the total number of optical signals in the images having the one or more predetermined characteristics is then determined.
Claims48
A method for analyzing one or more three dimensional spaces, comprising, for each three dimensional space:
- (a) for each of the one or more DNA sequences,
- (aa) labeling copies of the DNA sequence in the space with a probe producing a detectable optical signal;
- (ab) for each of a plurality of parallel planes passing through the space,
- (aba) obtaining a two-dimensional image of the space that is focused in the plane;
- and
- (abb) determining the number of optical signals from the DNA sequence in the image having one or more predetermining characteristics;
- and
- (ac) determining the total number of optical signals in the images having the one or more predetermined characteristics.
A system for analyzing one or more three-dimensional spaces, comprising:
- (a) an optical system configured, for each of a pluraity of parallel planes passing through a three-dimensional space, to obtain a two-dimensional image of the three dimensional space that is focused in the plane;
- and
- (b) a processor configured to:
- (ba) for each of a plurality of two-dimensional images of a three-dimensional space, to determine the number of optical signals from a DNA sequence in the image having one or more predetermined characteristics when the DNA sequence is labeled with a probe producing the optical signal;
- (bb) to determine the total number of optical signals from the DNA sequence in the images having the one or more predetermined characteristics.
A method for optimizing a classifier, the classifier being configured, for each of N quantifiable features, to determine a value of the feature associated with an object, the method comprising:
- (a) Determining a number n of features, n being n?N, that minimizes a classifier probability function of miscalculation;
- and
- (b) selecting n features from among the N features that maximize a ratio of between-class scatter to the within-class scatter.
A method for analyzing one or more three dimensional spaces, comprising, for each three dimensional space:
- (a) for each of the one or more DNA sequences,
- (aa) labeling copies of the DNA sequence in the space with a probe producing a detectable optical signal;
- (ab) for each of a plurality of parallel planes passing through the space,
- (aba) obtaining a two-dimensional image of the space that is focused in the plane;
- and
- (abb) determining the number of optical signals from the DNA sequence in the image having one or more predetermined characteristics;
- and
- (ac) determining the total number of optical signals in the images having the one or more predetermined characteristic.
A system for analyzing one or more three-dimensional spaces, comprising:
- (a) an optical system configured, for each of a plurality of parallel planes passing through a three-dimensional space, to obtain a two-dimensional image of the three dimensional space that is focused in the plane;
- and
- (b) a processor configured to:
- (ba) for each of a plurality of two-dimensional images of a three-dimensional space, to determine the number of optical signals from a DNA sequence in the image having one or more predetermined characteristics when the DNA sequence is labeled with a probe producing the optical signal;
- (bb) to determine the total number of optical signals from the DNA sequence in the images having the one or more predetermined characteristics.
A method for optimizing a classifier, the classifier being configured, for each of N quantifiable features, to determine a value of the feature associated with an object, the method comprising:
- (a) Determining a number n of features, n being n?N, that minimizes a classifier probability function of miscalculation;
- and
- (b) selecting n features from among the N features that maximize a ratio of between-class scatter to the within-class scatter.
Dependencies are inferred from the claim text.