Physalia fluorescent proteins
AssigneeUniversity of Hawaii
InventorAngel Anne Yanagihara
Life of this patent
Estimating the term…
Abstract
Provided are Physalia fluorescent proteins (PFPs) and, more particularly, to PFPs of a Physalia species, and methods of detecting and isolating PFPs. Also provided are methods and compositions for using PFPs, including recombinant PFPs, as reporter molecules in in vitro and in vivo biological assays, including screening assays and cellular assays.
Claims24
An isolated Physalia fluorescent protein (PFP) characterized by an excitation wavelength in a range of about 210-550 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength in a range of about 400-950 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength in a range of about 400-950 nm, when said PFP is excited at a wavelength in a range of about 210-550 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 456 nm, when said PFP is excited at an excitation wavelength of 221 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 421 nm, when said PFP is excited at an excitation wavelength of 222 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 600 nm, when said PFP excited at an excitation wavelength of 222 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 597 nm, when said PFP is excited at an excitation wavelength of 301 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 598 nm, when said PFP is excited at an excitation wavelength of 301 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 600 nm, when said PFP is excited at an excitation wavelength of 301 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength in a range of about 888-898 nm, when said PFP is excited at an excitation wavelength of 301 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 888 nm, when said PFP is excited at an excitation wavelength of 301 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 838 nm, when said PFP is excited at an excitation wavelength of 421 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 900 nm, when said PFP is excited at an excitation wavelength of 450 nm.
An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 898 nm, when said PFP is excited at an excitation wavelength of 451 nm.
A method for detecting a Physalia fluorescent protein (PFP), said method comprising:
- a) providing a sample comprising a Physalia fluorescent protein (PFP);
- b) applying to said sample a light source having an excitation wavelength in the range of about 210-550 nm, and
- c) detecting an emission wavelength in a range of about 400-950 nm,
- wherein said emission wavelength is indicative of said PFP.
A method for detecting a Physalia fluorescent protein (PFP), said method comprising:
- a) providing a sample comprising a Physalia fluorescent protein (PFP);
- b) applying a light source having an excitation wavelength within the excitation spectrum of said PFP, thereby exciting said PFP;
- and
- c) detecting an emission wavelength selected from a group of emission wavelengths consisting of:
- an emission wavelength of about 456 nm, when said PFP is excited by an excitation wavelength of 221 nm;
- an emission wavelength of about 421 nm, when said PFP is excited by an excitation wavelength of 222 nm;
- an emission wavelength of about 600 nm, when said PFP is excited by an excitation wavelength of 222 nm;
- an emission wavelength of about 597 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 598 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 600 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength in a range of about 888-898 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 888 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 838 nm, when said PFP is excited by an excitation wavelength of 421 nm;
- an emission wavelength of about 900 nm, when said PFP is excited by an excitation wavelength of 450 nm;
- and
- an emission wavelength of about 898 nm, when said PFP is excited by an excitation wavelength of 451 nm,
- wherein said emission wavelength is indicative of said PFP.
A method for isolating a Physalia fluorescent protein (PFP), said method comprising:
- a) providing a sample comprising a Physalia fluorescent protein (PFP);
- b) applying to said sample a light source having an excitation wavelength in the range of about 210-550 nm;
- c) detecting an emission wavelength in a range of about 400-950 nm,
- wherein said emission wavelength is indicative of said PFP;
- and
- d) selecting said PFP based on said emission wavelength.
A method for isolating a Physalia fluorescent protein (PFP), said method comprising:
- a) providing a sample comprising a Physalia fluorescent protein (PFP);
- b) applying a light source having an excitation wavelength within the excitation spectrum of said PFP, thereby exciting said PFP;
- and
- c) detecting an emission wavelength selected from a group of emission wavelengths consisting of:
- an emission wavelength of about 456 nm, when said PFP is excited by an excitation wavelength of 221 nm;
- an emission wavelength of about 421 nm, when said PFP is excited by an excitation wavelength of 222 nm;
- an emission wavelength of about 600 nm, when said PFP is excited by an excitation wavelength of 222 nm;
- an emission wavelength of about 597 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 598 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 600 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength in a range of about 888-898 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 888 nm, when said PFP is excited by an excitation wavelength of 301 nm;
- an emission wavelength of about 838 nm, when said PFP is excited by an excitation wavelength of 421 nm;
- an emission wavelength of about 900 nm, when said PFP is excited by an excitation wavelength of 450 nm;
- and
- an emission wavelength of about 898 nm, when said PFP is excited by an excitation wavelength of 451 nm,
- wherein said emission wavelength is indicative of said PFP;
- and
- d) selecting said PFP based on said emission wavelength.
Dependencies are inferred from the claim text.