Polynucleotides encoding antigenic HIV type C polypeptides, polypeptides and uses thereof
AssigneeChiron Corporation
InventorsJan zur Megede, Susan Barnett, Ying Lian, Susan Engelbrecht, Estrelita Janse van Rensburg
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Abstract
The present invention relates to polynucleotides encoding immunogenic HIV polypeptides. Uses of the polynucleotides in applications including immunization, generation of packaging cell lines, and production of HIV polypeptides are also described. Polynucleotides encoding antigenic HIV polypeptides are described, as are uses of these polynucleotides and polypeptide products therefrom, including formulations of immunogenic compositions and uses thereof.
Claims61
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Gag polypeptide, wherein the polynucleotide sequence encoding said Gag polypeptide comprises a sequence having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:
- 10, SEQ ID NO:11, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18 and SEQ ID NO:19.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Gag polypeptide, wherein the polynucleotide sequence encoding said Gag polypeptide comprises a sequence having at least 90% sequence identity to at least 500 contiguous nucleotides of SEQ ID NO:
- 12 or SEQ ID NO:20.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Env polypeptide, wherein the polynucleotide sequence encoding said Env polypeptide comprises a sequence having at least 90% sequence identity to SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29 and SEQ ID NO:30.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Env polypeptide, wherein the polynucleotide sequence encoding said Env polypeptide comprises a sequence having at least 90% sequence identity to SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, and SEQ ID NO:38.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Int polypeptide, wherein the polynucleotide sequence encoding said Int polypeptide comprises a sequence having at least 95% sequence identity to SEQ ID NO:39.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Int polypeptide, wherein the polynucleotide sequence encoding said Int polypeptide comprises a sequence having at least 98% sequence identity to SEQ ID NO:40.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Nef polypeptide, wherein the polynucleotide sequence encoding said Nef polypeptide comprises a sequence having at least 90% sequence identity to SEQ ID NO:41 or SEQ ID NO:203.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV plSRNaseH polypeptide, wherein the polynucleotide sequence encoding said pI5RNaseH polypeptide comprises a sequence having at least 90% sequence identity to SEQ ID NO:42.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Pol polypeptide, wherein the polynucleotide sequence encoding said Pol polypeptide comprises a sequence having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NO:43, SEQ ID NO:44 and SEQ ID NO:45.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Tat polypeptide, wherein the polynucleotide sequence encoding said Tat polypeptide comprises a sequence having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47 and SEQ ID NO:48.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Prot polypeptide, wherein the polynucleotide sequence encoding said Prot polypeptide comprises a sequence having at least 95% sequence identity to SEQ ID NO:49 or SEQ ID NO:50.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Prot polypeptide, wherein the polynucleotide sequence encoding said Prot polypeptide comprises a sequence having at least 90% sequence identity to SEQ ID NO:51.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Rev polypeptide, wherein the polynucleotide sequence encoding said Rev polypeptide comprises a sequence having at least 90% sequence identity to SEQ ID NO:52.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Tat polypeptide, wherein the polynucleotide sequence encoding said Tat polypeptide comprises a sequence having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, and SEQ ID NO:60.
An expression cassette, comprising a polynucleotide sequence encoding a polypeptide including an HIV Env polypeptide, wherein the polynucleotide sequence encoding said Env polypeptide comprises a sequence having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 183, SEQ ID NO:
- 184, SEQ ID NO:
- 185, SEQ ID NO:
- 186, SEQ ID NO:
- 187, SEQ ID NO:
- 188, SEQ ID NO:189, SEQ ID NO:190 and SEQ ID NO:191.
Dependencies are inferred from the claim text.