Prostase protein vaccine comprising derivatised thiol residues and methods for producing said antigen

A vaccine as claimed herein for use in medicine.

Use of a protein as claimed herein for the manufacture of a vaccine for immunotherapeutically treating a patient suffering from prostate cancer or other prostase-associated tumours.

A process for the purification of a prostase antigen, fragment, fusion or homologue thereof, comprising solubilising the protein utilising a combination of both a strong chaotropic agent and a detergent.

A process for the production of a a prostase antigen, fragment, fusion or homologue thereof, comprising the steps of treating the protein to reduce the protein's intra- and inter-molecular disulphide bonds, and blocking the thiol to prevent oxidative recoupling.

A process for the purification of a prostase antigen, fragment, fusion or homologue thereof comprising solubilising said antigen, fragment, fusion or homologue, reducing at least one of the protein's intra- and inter-molecular disulfide bond utilising a reducing agent, filtering the product, and blocking the resulting free thiol group.