Methods for selectively modulating survivin apoptosis pathways
AssigneeYale University
InventorDario C. Altieri
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Abstract
The present invention, based on the discovery of a new biological phenomena, provides methods and compositions for use in identifying agents that modulate the phosphorylation of survivin, the interaction between survivin and p34cdc2-cyclin B1 kinase complex, and the interaction between survivin and caspase-9. Related methods and compositions can be used to modulate survivin regulated apoptosis.
Claims29
A method of identifying an agent which modulates phosphorylation of survivin comprising the steps of:
- (a) incubating survivin and p34cdc2-cyclin B1 kinase complex in the presence of an agent;
- and
- (b) determining whether the agent modulates the phosphorylation of survivin by p34cdc2-cyclin B1 kinase complex, thereby identifying an agent which modulates the phosphorylation of surivin.
A method of modulating the interaction between survivin and p34cdc2 cyclin B1 kinase complex comprising the step of:
- administering an effective amount of an agent which modulates the interaction between survivin and p34cdc2-cyclin B1 kinase complex.
A method of modulating survivin dependent apoptosis in a cell, comprising the step of:
- administering to the cell an effective amount of an agent which modulates the interaction between survivin and p34cdc2-cyclin B1 kinase complex.
A method of modulating apoptosis in a cell, comprising the step of administering to the cell an effective amount of an agent which modulates the phosphorylation of survivin by p34cdc2-cyclin B1 kinase complex.
A method of reducing the severity of a pathological state mediated by survivin comprising the step of inhibiting the phosphorylation of survivin by p34cdc2-cyclin B1 kinase complex or the binding of survivin to p34cdc2-cyclin B1 kinase complex.
A method of identifying an agent which modulates the interaction between phosphorylated survivin and caspase-9 comprising the steps of:
- (a) incubating survivin and caspase-9 in the presence of an agent;
- and
- (b) determining whether the agent modulates the interaction of survivin and caspase-9, thereby identifying an agent which modulates the interaction between phosphorylated survivin and caspase-9.
A method of modulating the interaction between phosphorylated survivin and caspase-9 comprising the step of:
- administering an effective amount of an agent which modulates the interaction between survivin and caspase-9.
A method of modulating survivin dependent apoptosis in a cell, comprising the step of:
- administering to the cell an effective amount of an agent which modulates the interaction between phosphorylation survivin and caspase-9.
A method of reducing the severity of a pathological state mediated by survivin comprising the step of inhibiting the binding of survivin to caspase-9
A method of inhibiting the growth of tumor comprising administering an effective amount of a survivin antagonist to the tumor.
An antibody that binds to survivin phosphorylated at position 34.
Dependencies are inferred from the claim text.