Protease-activated receptor PAR4 (ZCHEMR2)
AssigneeZymoGenetics, Inc.
InventorsWenfeng Xu, Scott R. Presnell, David P. Yee, Donald C. Foster
Life of this patent
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Abstract
The present invention relates to polynucleotide and polypeptide molecules for PAR4, a novel member of the protease-activated receptor family. The polypeptides, and polynucleotides encoding them, mediate biological responses and/or cellular signaling in response to proteases. Protease cleavage of PAR4 exposes a PAR4 extracellular amino terminal portion that serves as a ligand for the PAR4 receptor. PAR4 may be used as a target in drug screening, and further used to identify proteinaceous or non-proteinaceous PAR4 agonists and antagonists. The present invention also includes antibodies to the PAR4 polypeptides.
Claims11
An isolated polynucleotide molecule encoding a PAR4 polypeptide selected from the group consisting of:
- a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 176 to nucleotide 1330;
- b) allelic variants of (a);
- c) orthologs of (a);
- and
- d) degenerate nucleotide sequences of (a), (b) or (c).
An isolated polynucleotide molecule encoding a PAR4 ligand selected from the group consisting of:
- a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 317 to nucleotide 409;
- b) allelic variants of (a);
- c) orthologs of (a);
- and
- d) degenerate nucleotide sequences of (a), (b) or (c).
An expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment selected from the group consisting of:
- a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 176 to nucleotide 1330;
- b) allelic variants of (a);
- c) orthologs of (a);
- and
- d) degenerate nucleotide sequences of (a), (b) or (c);
- and
- a transcription terminator.
An isolated PAR4 polypeptide.
An isolated PAR4 polypeptide selected from the group consisting of:
- a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 18 (Gly) to residue 385 (Gln);
- b) allelic variants of (a);
- and
- c) orthologs of (a),
- wherein the PAR4 polypeptide is a protease-activated receptor.
An isolated PAR4 ligand selected from the group consisting of:
- a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 48 (Gly) to residue 53 (Val);
- b) allelic variants of (a);
- and
- c) orthologs of (a).
Dependencies are inferred from the claim text.