Corneal cells expressing active agents and methods of use thereof
OwnerKeryn Anne Williams, Douglas John Coster +1inventors
InventorsKeryn Anne Williams, Douglas John Coster, Sonja Klebe
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Abstract
The invention relates to methods of modifying cells of corneal tissue to express an active agent, to modified corneal tissue, to vectors utilised in such methods and to methods of xeno- and allo-transplantation utilising the modified corneal tissue. The method of modifying cells of corneal tissue to express an active agent involves exposing harvested corneal tissue to an effective concentration for transfection of an expression vector which comprises a nucleotide sequence encoding for the active agent for a period sufficient to allow infection, such that cells of said corneal tissue will express the active agent.
Claims40
A method of modifying cells of corneal tissue to express an active agent comprising exposing harvested corneal tissue to an effective concentration for transfection of an expression vector which comprises a nucleotide sequence encoding for the active agent for a period sufficient to allow transfection, such that cells of said corneal tissue will express the active agent.
A corneal tissue comprising cells modified to express an active agent which is not expressed by normal corneal tissue or which following modification is expressed at elevated levels relative to normal corneal tissue.
A corneal tissue comprising cells modified to express an active agent, wherein modification is by exposing harvested corneal tissue to an effective concentration for infection of an expression vector which comprises a nucleotide sequence encoding for the active agent, for a period sufficient to allow infection.
A method of improving corneal graft healing and/or prolonging graft survival comprising exposing harvested corneal tissue to an effective concentration for infection of an expression vector which comprises a nucleotide sequence encoding for an active agent for a period sufficient to allow infection, such that cells of said corneal tissue will express the active agent, and then transplanting the corneal tissue to an eye of a recipient.
An expression vector for use in modifying corneal tissue to express an active agent not expressed by normal corneal tissue or which following modification is expressed at elevated levels relative to normal corneal tissue;
- the vector comprising a nucleotide sequence encoding for the active agent.
Dependencies are inferred from the claim text.