Recombinant adenoviruses expressing interleukin-18 protein and gene therapy using them
AssigneesSamyang Genex Corporation, Korea Research Institute of Bioscience and Biotechnology
InventorsDong-Soo Im, Won-Kyung Cho, Kyung-Sun Hwang, Young-Rim Seong, Seung-Suh Hong
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Abstract
The present invention relates to recombinant adenoviruses expressing interleukin-18 protein, and gene therapy using them. More particularly, the invention provides recombinant adenoviruses Ad.promIL-18, Ad.GMmIL-18, Ad.prohIL-18, Ad.hIL-18CPP32- and Ad.preprotrypsin.hIL-18CPP32- which are effectively capable of treating a variety of cancer cells by promoting and enhancing an immune response in vivo.
Claims32
An interleukin-18 protein wherein a cleavage site for caspase-3 is substituted by an amino acid selected from the group consisting of alanine, arginine, asparagine, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.
An interleukin-18 gene comprising a nucleotide sequence encoding the interleukin-18 protein, wherein cleavage site for caspase-3 of the interleukin-18 protein is substituted by an amino acid selected from the group consisting of alanine, arginine, asparagine, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.
A polynucleotide comprising a mature interleukin-18 gene and a signal sequence for extracellular secretion.
A recombinant adenovirus comprising
- (a) a promoter operable linked to an interleukin-18 gene so that it can be expressed,
- (b) the interleukin-18 gene, and
- (c) a polyadenylation signal sequence.
Dependencies are inferred from the claim text.