Porcine adenovirus E1 region
OwnerSuresh K. Tikooinventor
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Abstract
The present invention relates to the characterization of the porcine adenovirus E1 region. The complete nucleotide sequence of the genome of porcine adenovirus type 3 (PAV-3), providing the characterization of the PAV3 E1 region, is described herein. Methods for construction of infectious PAV genomes by homologous recombination in procaryotic cells are provided. Recombinant PAV viruses are obtained by transfection of mammalian cells with recombinant PAV genomes. The PAV-3 genome can be used as a vector for the expression of heterologous nucleotide sequences, for example, for the preparation and administration of subunit vaccines to swine or other mammals.
Claims47
A replication-defective recombinant PAV vector, comprising at least one heterologous nucleotide sequence, wherein the PAV vector lacks E1 A function and retains E1Bsmall function.
A replication-defective recombinant PAV vector, comprising at least one heterologous nucleotide sequence, wherein the PAV vector lacks E1Blarge function and retains E1Bsmall function.
A replication-defective recombinant PAV vector comprising at least one heterologous nucleotide sequence, wherein the PAV vector lacks E1A function and E1Bsmall function and retains E1large function.
A recombinant PAV vector comprising at least one heterologous nucleotide sequence, wherein said vector lacks E1Bsmall function and retains E1A and E1Blarge function.
A recombinant mammalian cell line that comprises nucleic acid encoding mammalian adenovirus E1A function and lacks nucleic acid encoding mammalian adenovirus E1Bsmall function.
A recombinant mammalian cell line that comprises nucleic acid encoding mammalian adenovirus E1Blarge function and lacks nucleic acid encoding mammalian adenovirus E1Bsmall function.
A method for producing a recombinant PAV, the method comprising:
- (a) introducing, into an appropriate helper cell line, a porcine adenovirus vector comprising ITR sequences, PAV packaging sequences, and at least one heterologous nucleotide sequence, wherein said vector lacks E1A function and retains E1Bsmall function;
- (b) culturing the cell line under conditions whereby adenovirus virus replication and packaging occurs;
- and
- (c) recovering the adenovirus from the infected cells.
Dependencies are inferred from the claim text.