Highly purified cytokine activating factor and methods of use
AssigneeDCV, Inc.
InventorsSubramanian Iyer, Tay N. Nguyen, Dauh-Rurng Wu, Ruye Xing
Life of this patent
Estimating the term…
Abstract
Disclosed is an isolated Cytokine Activating Factor (CAF) protein, an isolated nucleic acid molecule encoding a CAF protein, an antibody that selectively binds to a CAF protein, a composition comprising a Cytokine Activating Factor (CAF), and a method for modulating the immune system using the composition. Also disclosed is a method for treating cancer using such a composition.
Claims46
An isolated protein comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:6;
- and,
- b. an amino acid sequence comprising at least 9 consecutive amino acid residues of an amino acid sequence of (a);
- wherein said isolated protein upregulates expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β) or interleukin-6 (IL-6), or downregulates expression of transforming growth factor β (TGFβ).
An isolated protein comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:6;
- and,
- b. an amino acid sequence that is at least 65% identical to an amino acid sequence of (a) over at least 15 amino acids of an amino acid sequence of (a);
- wherein said isolated protein upregulates expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β) or interleukin-6 (IL-6), or downregulates expression of transforming growth factor β (TGFβ).
A composition comprising a pharmaceutically acceptable carrier and a cytokine activating factor (CAF) protein comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:6;
- and,
- b. an amino acid sequence comprising at least 9 consecutive amino acid residues of an amino acid sequence of (a);
- wherein said CAF protein upregulates expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β) or interleukin-6 (IL-6), or downregulates expression of transforming growth factor β (TGFβ).
A method to regulate an immune response in an animal, comprising administering to said animal a composition comprising a cytokine activating factor (CAF) protein comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:6;
- and,
- b. an amino acid sequence comprising at least 9 consecutive amino acid residues of an amino acid sequence of (a);
- wherein said CAF protein upregulates expression of tumor necrosis factor α (TNFα), interleukin-1β(IL-1β) or interleukin-6 (IL-6), or downregulates expression of transforming growth factor β (TGFβ).
A method of treating cancer in an animal, comprising administering to an animal that has or is at risk of developing cancer a composition comprising a cytokine activating factor (CAF) protein comprising an amino acid sequence selected from the group consisting of:
- a. an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:6;
- and,
- b. an amino acid sequence comprising at least 9 consecutive amino acid residues of an amino acid sequence of (a);
- wherein said CAF protein upregulates expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β) or interleukin-6 (IL-6), or downregulates expression of transforming growth factor β (TGFβ).
An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
- a. a nucleic acid sequence encoding a protein comprising an amino acid sequence selected from the group consisting of:
- i. an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:6;
- and,
- ii. an amino acid sequence comprising at least 9 consecutive amino acid residues of an amino acid sequence of (i);
- wherein said protein upregulates expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β) or interleukin-6 (IL-6), or downregulates expression of transforming growth factor β (TGFβ);
- and,
- b. a nucleic acid sequence that is fully complementary to said nucleic acid sequence of (a).
Dependencies are inferred from the claim text.