Fibrin binding moieties useful as imaging agents
AssigneeDyax Corp.
InventorsCharles R. Wescott, James P. Beltzer, Aaron K. Sato
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Abstract
The present invention provides binding moieties for fibrin which have a variety of uses wherever detecting, isolating or localizing fibrin, and particularly fibrin as opposed to fibrinogen, is advantageous. Particularly disclosed are synthetic, isolated polypeptides capable of binding fibrin and recognizing the form of polymerized fibrin found in thrombi. In addition, the polypeptides have a slow dissociation rate from fibrin, which improves their ability to form a contrast image at the site of a fibrin clot, making the disclosed binding moieties particularly useful as imaging agents for thrombi.
Claims35
An isolated polypeptide having the ability to bind to fibrin comprising the amino acid sequence:
- Cys-X2-X3-X4-X5-X6-X7-X8-Cys (SEQ ID NO:
- 2), wherein
- X2 is Pro, Arg, Asn, Asp, Gln, Gly, Phe, Ser, Thr or Tyr;
- X3 is Ala, Asn, Asp, Gln, Glu, Gly, Ile, Leu, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- X4 is Glu, Gly, Lys, Ser, or Tyr;
- X5 is Pro, Asp, Glu, Asn, Gln, Glu, Gly, Leu, Lys, Ser, Thr, or Tyr;
- X6 is Arg, Gly, or Trp;
- X7 is Leu, Ile, Lys, Met, Asn, Gln, Pro, Ser, Thr, or Val;
- and
- X8 is Ile, Leu, Phe, Trp, Tyr.
A fibrin binding polypeptide comprising the amino acid sequence:
- Cys-Asp-Tyr-Tyr-Gly-Thr-Cys (SEQ ID NO:
- 26).
Dependencies are inferred from the claim text.