Electrophoresis gel assembly and method of assembling the same
AssigneeGrace Ventures LLC
InventorsWilliam F. Alpenfels, Marilyn Alpenfels
Life of this patent
Estimating the term…
Abstract
A thin-film electrophoresis assembly includes a support frame having a top portion and a substantially flat face, a first thin-film member carried by the support frame, a second thin-film member carried by the support frame, and a resolving gel sandwiched between the first thin-film member and the second thin-film member. A reservoir is mounted to the top portion of the support frame and includes an open top and an open bottom. A stacking gel is carried by the reservoir and includes a bottom surface in fluid-tight communication with a top surface of the resolving gel through the open bottom of the reservoir.
Claims16
A thin-film, electrophoresis assembly, comprising:
- a support frame including a top portion and a substantially flat face;
- a first thin-film member carried by the support frame;
- a second thin-film member carried by the support frame;
- a resolving gel sandwiched between the first thin-film member and the second thin-film member, the resolving gel including a top surface;
- a reservoir mounted to the top portion of the support frame, the reservoir including an open top and an open bottom;
- and a stacking gel carried by the reservoir and including a bottom surface in fluid-tight communication with the top surface of the resolving gel through the open bottom of the reservoir.
A support frame for an electrophoresis assembly including a first thin-film member, a second thin-film member, an electrophoresis gel there between, and a reservoir including front and rear protruding members that extend outward and laterally from the reservoir, the support frame comprising:
- a substantially rectangular support frame having an interconnected upper lateral support, a lower lateral support, a left vertical support, a right vertical support, and ear-like, flat protrusions that extend from the support frame near intersections of the interconnected upper lateral support with the vertical supports, the ear-like, flat protrusions slidably received by the front and rear protruding members to mount the reservoir on top of the support frame.
A reservoir for an electrophoresis assembly including a support frame that carries an electrophoresis gel sandwiched between a first thin-film member and a second thin-film member, the support frame including ear-like, flat protrusions that extend upwardly from a top portion of the support frame, the reservoir comprising:
- a trough-shaped member including an open top and an open bottom, the reservoir decreasing in thickness from the open top to the open bottom, front and rear protruding members extending outward and laterally from the reservoir, the front and rear protruding members slidably receiving the ear-like, flat protrusions of the support frame to mount the reservoir on top of the support frame.
A method of assembling an electrophoresis assembly, comprising:
- providing a substantially rectangular support frame having an interconnected upper lateral support, a lower lateral support, a left vertical support, a right vertical support, and ear-like, flat protrusions that extend from the support frame near intersections of the interconnected upper lateral support with the vertical supports, the support frame carrying an electrophoresis gel sandwiched between a first thin-film member and a second thin-film member;
- providing a reservoir including a trough-shaped member including an open top and an open bottom, the reservoir decreasing in thickness from the open top to the open bottom, front and rear protruding members extending outward and laterally from the reservoir, mounting the reservoir to the support frame by sliding the front and rear protruding members of the reservoir over the ear-like, flat protrusions of the support frame.
A method of assembling an electrophoresis assembly, comprising:
- separately casting a stacking gel and a resolving gel;
- providing the stacking gel in a reservoir having an open bottom;
- providing the resolving gel between two thin-film members on a support frame, the resolving gel having a top edge and a perimeter that is not sealed;
- and joining the stacking gel with the top edge of the resolving gel through the open bottom of the reservoir.
Dependencies are inferred from the claim text.