Production of polysaccharide
AssigneesShin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
InventorThomas J. Pollock
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Abstract
The present invention provides for a polysaccharide produced by subjecting a Sphingomonas bacterium modified with a S7c6 gene cluster or segment thereof to aerobic fermentation in a nutrient aqueous broth for a time sufficient to produce the polysaccharide dissolved therein, including L-Rhap, D-Glcp and 2-deoxy-β-D-arabino-HexpA in a molar ratio of 1:3:1, wherein the polysaccharide has at least 20% less glucose per repeat unit compared to a heteropolysaccharide S-7 produced by an unmodified Sphingomonas strain S7, and the segment includes at least the spsB and rhsACBD genes.
Claims12
A polysaccharide produced by subjecting a Sphingomonas bacterium modified with a S7c6 gene cluster or segment thereof to aerobic fermentation in a nutrient aqueous broth for a time sufficient to produce the polysaccharide dissolved therein, the polysaccharide comprising L-Rhap, D-Glcp and 2-deoxy-β-D-arabino-HexpA in a molar ratio of 1:3:1, wherein the polysaccharide has at least 20% less glucose per repeat unit compared to a heteropolysaccharide S-7 produced by an unmodified Sphingomonas strain S7, and the segment comprises at least the spsB and rhsACBD genes.
A method for increasing the viscosity of an aqueous solution comprising adding to the aqueous solution a viscosity increasing effective amount of a polysaccharide comprising L-Rhap, D-Glcp and 2-deoxy-β-D-arabino-HexpA in a molar ratio of 1:3:1, wherein the polysaccharide is produced by subjecting a Sphingomonas bacterium modified with a S7c6 gene cluster or segment thereof to aerobic fermentation in a nutrient aqueous broth for a time sufficient to produce the polysaccharide dissolved therein, and the segment comprises at least the spsB and rhsACBD genes.
A fermentation broth obtained by subjecting a Sphingomonas bacterium modified with a S7c6 gene cluster or segment thereof to aerobic fermentation in a nutrient aqueous broth for a time sufficient to produce a dissolved polysaccharide, wherein the polysaccharide comprises L-Rhap, D-Glcp and 2-deoxy-β-D-arabino-HexpA in a molar ratio of 1:3:1, the polysaccharide has at least 20% less glucose per repeat unit compared to a heteropolysaccharide S-7 produced by an unmodified Sphingomonas strain S7, and the segment comprises at least the spsB and rhsACBD genes.
Dependencies are inferred from the claim text.