Nucleic acids and proteins from group B streptococcus
AssigneeMicrobial Technics Limited
InventorsRichard William Falla Le Page, Jeremy Mark Wells, Sean Bosco Hanniffy
Life of this patent
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Abstract
Novel protein antigens from Group B Streptococcus are described, together with nucleic acid sequences encoding them. Their use in vaccines and screening methods is also described.
Claims23
A Group B Streptococcus protein having a sequence selected from those described in FIG. 1, or fragments or derivatives thereof.
A Group B Streptococcus polypeptide or peptide having a sequence selected from those described in FIG. 2, or fragments or derivatives thereof.
A nucleic molecule comprising or consisting of a sequence which is:
- (i) any of the DNA sequences set out in FIG. 1 and FIG. 2 herein or their RNA equivalents;
- (ii) a sequence which is complementary to any of the sequences of (i);
- (iii) a sequence which codes for the same protein or polypeptide, as those sequences of (i) or (ii);
- (iv) a sequence which shows substantial identity with any of those of (i), (ii) and (iii);
- or
- (v) a sequence which codes for a derivative, or fragment of a nucleic acid molecule shown in FIG. 1 or FIG. 2.
A method of detection of Group B Streptococcus which comprises the step of bringing into contact a sample to be tested with at least one antibody, affibody, or a derivative thereof, as described herein.
A method of detection of Group B Streptococcus which comprises the step of bringing into contact a sample to be tested with at least one protein, polypeptide, peptide, fragments or derivatives as described herein.
A method of detection of Group B Streptococcus which comprises the step of bringing into contact a sample to be tested with at least one nucleic acid molecule as described herein.
A method of screening for DNA encoding bacterial cell envelope associated or surface antigens in gram positive bacteria comprising the steps of:
- combining a reporter vector including the nucleotide sequence encoding the mature from of the staphylcoccus nuclease gene and an upstream promoter region with DNA from a gram positive bacteria.
- transforming the resultant vector into Lactococcus lactis cells.
- assaying for the secretion of staphlycoccus nuclease protein in the transformed cells.
A vector as shown in FIG. 4 for use in screening for DNA encoding bacterial cell envelope associated or secreted antigens in gram positive bacteria.
Dependencies are inferred from the claim text.