Nuclear fertility restorer genes and methods of use in plants
AssigneesMcGill University, DNA Landmarks, Inc.
InventorsGregory G. Brown, Natasa Formanova, Charles Dendy, Benoit S. Landry, Wing Cheung, Hua Jin
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Abstract
The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.
Claims51
An isolated nuclear fertility restorer nucleic acid selected from the group consisting of:
- a) a nucleic acid comprising a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87;
- b) a nucleic acid encoding a protein comprising an amino acid sequence encoded by a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87;
- c) a nucleic acid comprising a nucleotide sequence differing from the sequence of the nucleic acids of a) or b) due to the degeneracy of the genetic code;
- d) a nucleic acid fragment of any of a) through c) above at least 150 nucleotides in length;
- and
- e) a nucleic acid having at least 70% homology with a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87, wherein sequence homology is determined by the Karlin and Altschul algorithm using standard parameters.
A first isolated nucleic acid that hybridizes under highly stringent conditions to a second nucleic acid selected from the group consisting of:
- a) a second nucleic acid comprising a nucleotide sequence selected from SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:52, SEQ ID NO:54 and SEQ ID NO:89;
- and
- b) a second nucleic acid encoding a protein comprising an amino acid sequence selected from SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:51, SEQ ID NO:53 and SEQ ID NO:88, wherein the first nucleic acid encodes a nuclear fertility restorer protein.
A method of producing a hybrid plant, comprising crossing a male-sterile plant with a male-fertile plant, collecting hybrid seed from the male-sterile plant and regenerating the hybrid plant from the seed, wherein the male-fertile plant comprises a nuclear fertility restorer nucleic acid selected from the group consisting of:
- a) a nucleic acid comprising a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87;
- b) a nucleic acid encoding a protein comprising an amino acid sequence encoded by a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87;
- c) a nucleic acid comprising a nucleotide sequence differing from the sequence of the nucleic acids of a) or b) due to the degeneracy of the genetic code;
- d) a nucleic acid fragment of any of a) through c) above at least 150 nucleotides in length;
- and
- e) a nucleic acid having at least 70% homology with a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87, wherein sequence homology is determined by the Karlin and Altschul algorithm using standard parameters.
A method of increasing production of viable pollen in a plant, comprising introducing a nuclear fertility restorer nucleic acid into the plant, wherein the nuclear fertility restorer nucleic acid selected from the group consisting of:
- a) a nucleic acid comprising a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87;
- b) a nucleic acid encoding a protein comprising an amino acid sequence encoded by a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87;
- c) a nucleic acid comprising a nucleotide sequence differing from the sequence of the nucleic acids of a) or b) due to the degeneracy of the genetic code;
- d) a nucleic acid fragment of any of a) through c) above at least 150 nucleotides in length;
- and
- e) a nucleic acid having at least 70% homology with a gene within positions 88,073 and 198,041 of the nucleotide sequence of SEQ ID NO:87, wherein sequence homology is determined by the Karlin and Altschul algorithm using standard parameters.
Dependencies are inferred from the claim text.