Alteration of embryo/endosperm size during seed development
OwnerRebecca E. Cahoon, Elmer P. Heppard +2inventors
InventorsRebecca E. Cahoon, Elmer P. Heppard, Nobuhiro Nagasawa, Hajime Sakai
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Abstract
Isolated nucleic acid fragments and recombinant constructs comprising such fragments for altering embryo/endosperm size during seed development are disclosed along with a method of controlling embryo/endosperm size during seed development in plants.
Claims39
An isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding a cytochrome P450 polypeptide associated with controlling embryo/endosperm size during seed development having an amino acid identity of at least 61% based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of SEQ ID NO:2, 7, 11, 19, 27, or 33;or
- (b) a nucleic acid sequence encoding a cytochrome P450 polypeptide associated with controlling embryo/endosperm size during seed development having an amino acid identity of at least 65% based on the Clustal method of alignment when compared to a third polypeptide selected from the group consisting of SEQ ID NO:15, 17, 31, 93, 95, 97, or 99;
- or
- (c) a nucleic acid sequence encoding a cytochrome P450 polypeptide associated with controlling embryo/endosperm size during seed development having an amino acid identity of at least 70% based on the Clustal method of alignment when compared to a fourth polypeptide selected from the group consisting of SEQ ID NO:9, 13, 23, 29, 35, or 4l;
- or
- (d) a nucleic acid sequence encoding a cytochrome P450 polypeptide associated with controlling embryo/endosperm size during seed development having an amino acid identity of at least 77% based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of SEQ ID NO:21, 25, 37, or 39;
- or
- (e) the complement of (a) or (b) or (c) or (d).
An isolated nucleic acid fragment comprising a promoter wherein said promoter consists essentially of the nucleotide sequence set forth in SEQ ID NOs:3, 4, 104, or 105, or said promoter consists essentially of a fragment or subfragment that is substantially similar and functionally equivalent to the nucleotide sequence set forth in SEQ ID NOs:3, 4, 104, or 105.
A method to isolate nucleic acid fragments encoding polypeptides associated with controlling embryo/endosperm size during seed development which comprises:
- (a) comparing SEQ ID NOs:2, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 42, 43, 44, 45, 46, 47, 93, 95, 97, or 99 with other polypeptide sequences associated with controlling embryo/endosperm size during seed development;
- (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);
- (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b);
- and
- (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with controlling embryo/endosperm size during seed development by sequence dependent protocols.
A method of mapping genetic variations related to controlling embryo/endosperm size and/or altering oil phenotype in plants comprising:
- (a) crossing two plant varieties;
- and
- (b) evaluating genetic variations with respect to
- (i) a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 92, 94, 96, 98, 100, 102, 104, or 105;
- or
- (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NO:2, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 42, 43, 44, 45, 46, 47, 80-91, 93, 95, 97, or 99;
- in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of:
- RFLP analysis, SNP analysis, and PCR-based analysis.
A method of molecular breeding to control embryo/endosperm size and/or altering oil phenotype in plants comprising:
- (a) crossing two plant varieties;
- and
- (b) evaluating genetic variations with respect to
- (i) a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 92, 94, 96, 98, 100, 102, 104, or 105;
- or
- (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NO:2, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 42, 43, 44, 45, 46, 47, 80-91, 93, 95, 97, or 99;
- in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of:
- RFLP analysis, SNP analysis, and PCR-based analysis.
Dependencies are inferred from the claim text.