Genes for plant fatty acid modifying enzymes associated with conjugated double bond formation
AssigneeE. I. Du Pont de Nemours and Company
InventorsEdgar Benjamin Cahoon, Thomas J. Carlson, William Dean Hitz, Kevin G. Ripp
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Abstract
The preparation and use of nucleic acid fragments encoding plant fatty acid modifying enzymes associated with conjugated double bond formation or functionally equivalent subfragments thereof are disclosed. Chimeric genes incorporating such nucleic acid fragments or functionally equivalent subfragments thereof or complement thereof and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles.
Claims62
An isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation wherein said fragment or a functionally equivalent subfragment thereof (a) hybridizes to any of the nucleotide sequences set forth in SEQ ID NOS:1, 3, 19, 23, or 29 under conditions of moderate stringency or (b) is at least 45% identical to a polypeptide encoded by any of the nucleotide sequences set forth in SEQ ID NOS:1, 3, 19, 23, or 29 or a functionally equivalent subfragment thereof as determined by a comparison method designed to detect homologous sequences.
An isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation wherein said fragment or a functionally equivalent subfragment thereof encodes a protein comprising any one of the amino acid sequences set forth in SEQ ID NOS:2, 4, 20, 24, or 30.
An isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation.
A method to isolate nucleic acid fragments and functionally equivalent subfragments thereof encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising:
- (a) comparing SEQ ID NOS:2, 4, 20, 24, and 30 and other plant fatty acid modifying enzyme polypeptide sequences;
- (b) identifying conserved sequences of 4 or more amino acids obtained in step (a);
- (c) designing degenerate oligomers based on the conserved sequences identified in step (b);
- and
- (d) using the degenerate oligomers of step (s) to isolate sequences encoding a plant fatty acid modifying enzyme or a portion thereof associated with conjugated double bond formation by sequence dependent protocols.
Animal feed comprising at least one conjugated linolenic acid derived from oil extracted from a natural source selected from the group consisting of tung, bittermelon, pot marigold, jacaranda, catalpa, and pomegranate.
Dependencies are inferred from the claim text.