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US 2003/0126638 A1Published application
Alteration of oil traits in plants
AssigneesE. I. Du Pont de Nemours and Company, Pioneer Hi-Bred International, Inc.
InventorsWilliam B. Allen, Rebecca E. Cahoon, Omolayo O. Famodu, Leslie T. Harvell, Timothy G. Helentjaris, Changjiang Li, Keith S. Lowe, Igor Cunha Oliveira, Bo Shen, Mitchell C. Tarczynski
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Abstract
The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
Claims21
An isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding a fifth polypeptide having Hap2-like transcription factor activity, the fifth polypeptide having at least 70% identity based on the Clustal method of alignment when compared to a sixth polypeptide selected from the group consisting of SEQ ID NOs:
- 2, 4, 5, 6, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, or 208, 210, 212, 214, or 216;
- or
- (b) a nucleic acid sequence encoding a seventh polypeptide having Hap5-like transcription factor activity, the seventh polypeptide having at least 80% identity based on the Clustal method of alignment when compared to an eighth polypeptide selected from the group consisting of SEQ ID NOs:
- 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, or 221;
- or
- (c) a nucleic acid sequence encoding a seventeenth polypeptide having Hap3/Lec1-like activity, the seventeenth polypeptide having at least 70% identity based on the Clustal method of alignment when compared to a eighteenth polypeptide selected from the group consisting of SEQ ID NOs:
- 130, 132, 134, or 136.
5 more elements
A method for altering oil phenotype in a plant which comprises:
- (a) transforming a plant with a recombinant DNA construct comprising an isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
- (i) a nucleic acid sequence encoding a plant Hap2-like transcription factor;
- (ii) the complement of the nucleic acid sequence of (i);
- (iii) the sequence of (i) or (ii) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
- (iv) a nucleic acid sequence encoding a plant Hap5-like transcription factor;
- (v) the complement of the nucleic acid sequence of (iv);
- (vi) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
- (vii) a nucleic acid sequence encoding a plant Hap3/Lec1 or Lec1-CCAAT transcription factor;
- (viii) the complement of the nucleic acid sequence of (vii);
- (ix) the sequence of (vii) or (viii) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
- wherein said nucleic acid sequence is operably linked to at least one regulatory sequence;
- (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct;
- and
- (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
11 more elements
A method for altering oil phenotype in a plant which comprises:
- (a) transforming a plant with a recombinant DNA construct comprising isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
- (i) a nucleic acid sequence encoding a plant Hap3/Lec1 transcription factor having at least 60% identity based on the Clustal method of alignment when compared to a fourth polypeptide selected from the group consisting of even SEQ ID NOs:
- from 130 to 148, and SEQ ID NOs:
- 195 and 196;
- (ii) the complement of the nucleic acid sequence of (i);
- (iii) the sequence of (i) or (ii) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
- (iv) a nucleic acid sequence encoding a plant Lec1-related CCAAT binding transcription factor having at least 60% identity base Clustal method of alignment when compared to a second polypeptide selected from the group consisting of even SEQ ID NOs:
- from 150 to 178, and SEQ ID NOs:
- 197 to 202;
- (v) the complement of the nucleic acid sequence of (iv);
- (vi) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
- wherein said nucleic acid sequence is operably linked to at least one regulatory sequence;
- (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct;
- and
- (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
12 more elements
A method to isolate nucleic acid fragments associated with altering oil phenotype in a plant which comprises:
- (a) comparing all even SEQ ID NOs:
- from 2 to 178, and 206 to 214, and SEQ ID NOs:
- 179 to 202, 216 to 219, 221, and 222 with other polypeptide sequences for the purpose of identifying polypeptides associated with altering oil phenotype in a plant;
- (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);
- (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b);
- and
- (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering oil phenotype by sequence dependent protocols.
4 more elements
A method for altering oil phenotype in a plant which comprises:
- (a) transforming a plant with a recombinant DNA construct comprising an isolated nucleic acid fragment operably linked to at least one regulatory sequence wherein said fragment has a nucleic acid sequence encoding a polypeptide having a sequence identity of at least 50% based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of even SEQ ID NOs:
- from 2 to 178, and 206 to 214, and SEQ ID NOs:
- 179 to 202, 216 to 219, 221, and 222;
- (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct;
- and
- (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
3 more elements
A method of mapping genetic variations related to altered oil phenotypes in a plant comprising:
- (a) crossing two plant varieties;
- and
- (b) evaluating genetic variations with respect to nucleic acid sequences set forth in any one of the odd SEQ ID NOs:
- from 1 to 177, or 207 to 215, or SEQ ID NO:
- 220 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of:
- RFLP analysis, SNP analysis, and PCR-based analysis.
3 more elements
A method of molecular breeding to obtain altered oil phenotypes in a plant comprising:
- (a) crossing two plant varieties;
- and
- (b) evaluating genetic variations with respect to nucleic acid sequences set forth in any one of the odd SEQ ID NOs:
- from 1 to 177, or 207 to 215, or SEQ ID NO:
- 220 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of:
- RFLP analysis, SNP analysis, and PCR-based analysis.
3 more elements
A method for altering oil phenotype in a plant which comprises:
- (a) transforming a plant with a recombinant DNA construct comprising isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
- (i) a nucleic acid sequence encoding a plant Hap3/Lec1 transcription factor having at least 70% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of SEQ ID NOs:
- 130 to 148, and SEQ ID NOs:
- 195, 196, and 206;
- (ii) the complement of the nucleic acid sequence of (iv);
- (iii) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
- (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct;
- and
- (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
6 more elements
A method to isolate nucleic acid fragments associated with altering oil phenotype in a plant which comprises:
- (a) comparing the even SEQ ID NOs:
- from 2 to 178, and 206 to 214, and SEQ ID NOs:
- 179 to 202, 216 to 219, 221, and 222, with other polypeptide sequences for the purpose of identifying polypeptides associated with altering oil phenotype in a plant;
- (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);
- (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b);
- and
- (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering oil phenotype by sequence dependent protocols.
4 more elements
Dependencies are inferred from the claim text.