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AssigneeCambria Biosciences, LLC
InventorsLeo Liu, Lucinda Burnam, Ann Sluder, Elizabeth Link, Bethany Westlund
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The invention provides a method of identifying anti-nematode compounds and further provides transgenic nematodes that may be used to practice the method. In particular, the invention provides a screen for compounds that inhibit a nematode secretion pathway e.g., compounds that inhibit the secretion of proteins by nematodes. The transgenic nematodes express reporters for nematode secreted proteins. In preferred embodiments of the invention the screen is performed using C. elegans, i.e., certain embodiments of the invention utilize C. elegans and C. elegans secretory pathways as a model system for parasitic nematodes and parasitic nematode secretion pathways. The invention also provides pharmaceutical compositions that may be used in the treatment and prevention of nematode infection in humans and animals and anti-nematode agents that may be used to protect plants from plant-parasitic nematodes. In addition, the invention provides a genetic screen for identifying additional targets for anti-nematode compounds.
A transgenic nematode, the cells of which contain a transgene comprising a regulatory element of a gene that encodes a nematode secretory product or a homolog thereof operably linked to a DNA sequence encoding a detectable marker.
A method of identifying a compound that inhibits a nematode secretion pathway comprising steps of:
A method of identifying a compound that inhibits a nematode secretion pathway comprising steps of:
A method of generating a nematode comprising steps of:
A method of identifying a target for anti-nematode compound development comprising the steps of:
A vector comprising a polynucleotide that encodes a polypeptide, the amino acid sequence of which comprises the sequence of SEQ ID NO:1.
A vector comprising a polynucleotide that encodes a polypeptide, the amino acid sequence of which comprises at least 10 consecutive residues of SEQ ID NO:1.
A vector comprising a polynucleotide that encodes a polypeptide, the amino acid sequence of which comprises a sequence at least 50% identical to SEQ ID NO:1.
A vector comprising a regulatory element comprising a polynucleotide sequence comprising the C. elegans vap-1 promoter operably linked to a polynucleotide encoding a detectable marker.
A vector comprising the polynucleotide sequence of SEQ ID NO:2.
A vector comprising a polynucleotide that encodes a polypeptide, the amino acid sequence of which comprises the sequence of SEQ ID NO:3.
The vector of claim 83, further comprising a polynucleotide sequence that encodes a detectable marker in frame with the polynucleotide sequence of claim 500.
A vector comprising a polynucleotide that encodes a polypeptide, the amino acid sequence of which comprises at least 10 consecutive residues of SEQ ID NO:3.
A vector comprising a polynucleotide that encodes a polypeptide, the amino acid sequence of which comprises a sequence at least 50% identical to SEQ ID NO:3.
A vector comprising a regulatory element comprising a polynucleotide sequence comprising the C. elegans vap-2 promoter operably linked to a polynucleotide encoding a detectable marker.
A vector comprising the polynucleotide sequence of SEQ ID NO:4.
A vector comprising a regulatory element comprising a polynucleotide sequence comprising the promoter of a gene belonging to the C. elegans vap family of genes operably linked to a polynucleotide encoding a detectable marker.
A method of expressing a first polynucleotide in a C. elegans amphid sheath cell comprising the step of:
A method of expressing a polypeptide in a C. elegans amphid sheath cell comprising the step of:
A method of expressing a first polynucleotide in a C. elegans amphid sheath cell comprising the step of:
A method of expressing a polypeptide in a C. elegans amphid sheath cell comprising the step of:
Dependencies are inferred from the claim text.