Methods for the regeneration of bone and cartilage
OwnerJackson Streeterinventor
Life of this patent
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Abstract
Therapeutic methods for regenerating bone and cartilage are described, the methods including delivering a tissue regenerative effective amount of light energy having a wavelength in the visible to near-infrared wavelength range to a site of injured or damaged bone or cartilage. The tissue regenerative effective amount of light energy is a predetermined power density (mW/cm2) received at the site, and is determined by selecting a dosage and power of the light energy sufficient to deliver the predetermined power density of light energy to the site of damage or injury. The light to methods are further applicable to in vitro or in vivo growth of cartilage replacement tissue on a biocompatible three-dimensional scaffold.
Claims67
A method for the regeneration of bone or cartilage in a subject in need of such treatment, said method comprising delivering a tissue regenerative effective amount of light energy having a wavelength in the visible to near-infrared wavelength range to a site in the bone or cartilage of the subject that includes an area of injury or damage wherein delivering the tissue regenerative effective amount of light energy comprises selecting a dosage and power of the light energy sufficient to deliver a predetermined power density of light energy to the site of at least about 0.01 mW/cm2.
A method for treating injury or damage of bone or cartilage comprising administering an isolated DNA molecule comprising a DNA sequence selected from known isolated gene sequences encoding gene products involved in osteogenesis or chondrogenesis to a subject in need osteogenesis or chondrogenesis, and delivering a tissue regenerative effective amount of light energy having a wavelength in the visible to near-infrared wavelength range to a site in the bone or cartilage of the subject that includes an area of injury or damage, wherein delivering the tissue regenerative effective amount of light energy includes selecting a power density of light energy to be delivered to the site of at least about 0.01 mW/cm2.
A method for treating injury or damage of bone or cartilage comprising administering a recombinant protein encoded by an isolated DNA molecule comprising a DNA sequence selected from known isolated gene sequences encoding gene products involved in osteogenesis or chondrogenesis to a subject in need of osteogenesis or chondrogenesis, and delivering a tissue regenerative effective amount of light energy having a wavelength in the visible to near-infrared wavelength range to a site in the bone or cartilage of the subject that includes an area of injury or damage, wherein delivering the tissue regenerative effective amount of light energy includes selecting a power density of light energy to be delivered to the site of at least about 0.01 mW/cm2.
A method for increasing the rate at which an implant or transplant prepared from cartilage cultured on three-dimensional scaffolding in vitro is integrated at a recipient site after transplantation or implantation, by delivering a tissue regenerative effective amount of light energy to the transplantation or implantation site wherein delivering a tissue regenerative effective amount of light energy includes selecting a power density (mW/cm2) of the light energy to be delivered to the culture of at least about 0.01 mW/cm2.
A method of producing cartilage at a cartilage defect site in vivo comprising:
- implanting into the defect site a biocompatible, non-living three-dimensional scaffold structure in combination with periosteal tissue, perichondrial tissue or a combination of periosteal and perichondrial tissues;
- separately administering into the defect site a preparation of stromal cells for attachment to the scaffold in vivo and for inducing chondrogenesis or migration of stromal cells from the in vivo environment adjacent to the defect site to the scaffold;
- and
- delivering a tissue regenerative effective amount of light energy to the defect site wherein delivering a tissue regenerative effective amount of light energy includes selecting a power density (mW/cm2) of the light energy to be delivered to the culture of at least about 0.01 mW/cm2.
A method for forming artificial cartilage, comprising:
- delivering a tissue regenerative effective amount of light energy to an in vitro culture comprising a preparation of stromal cells and a substrate for attachment of cells;
- and
- culturing the cells in a cell culture chamber for a time sufficient to produce artificial cartilage,
- wherein delivering a tissue regenerative effective amount of light energy includes delivering light having a wavelength in the visible to near-infrared wavelength range and a power density of at least about 0.01 mW/cm2 to the cells during culturing.
Dependencies are inferred from the claim text.