Trypsin substrate and diagnostic device, and method of using same
AssigneeBayer Aktiengesellschaft
InventorsPaul F. Corey, Steven W. Felman, Gary E. Rehm, Michael J. Pugia
Life of this patent
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Abstract
A compound of the formula (I) 1 (I) wherein R1 is a protecting group for Nα, R2 is a protecting group for NG, and R3 is aryl, and wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O—C single bond, which liberates R3—OH; a diagnostic device comprising same; a method for preparing the diagnostic device; and a method of using the diagnostic device to detect levels of urinary trypsin inhibitor in a biological sample; and a diagnostic kit for detecting levels of urinary trypsin inhibitor in a biological sample.
Claims41
A compound of the formula (I)
- 6
- wherein R1 is a protecting group for Nα;
- R2 is a protecting group for NG;
- and
- R3 is aryl;
- and
- wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O—C single bond, which liberates R3—OH.
A diagnostic device comprising:
- a carrier matrix;
- and
- a compound of the formula (I)
- 7
- wherein R1 is a protecting group for Nα;
- R2 is a protecting group for NG;
- and
- R3 is aryl;
- and
- wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O—C single bond, which liberates R3—OH.
A method of preparing a diagnostic device, the device comprising a carrier matrix and a trypsin substrate of formula (I)
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- wherein R1 is a protecting group for Nα;
- R2 is a protecting group for NG;
- and
- R3 is aryl;
- and
- wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O—C single bond, which liberates R3—OH;
- the method comprising:
- (a) contacting a carrier matrix with a buffer solution;
- (b) drying the carrier matrix;
- and
- (c) contacting the carrier matrix with a solution comprising the trypsin substrate of formula (I).
A method for detecting levels of urinary trypsin inhibitor in a biological sample comprising:
- contacting a biological sample with a predetermined amount of trypsin, a predetermined amount of a diazonium salt, and a diagnostic device comprising a trypsin substrate of the formula (I)
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- wherein R1 is a protecting group for Nα;
- R2 is a protecting group for NG;
- and
- R3 is aryl;
- and
- wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O—C single bond, which liberates R3—OH;
- and
- wherein the compound R3—OH reacts with a diazonium salt to form a visible color such that the greater the intensity of the color, the less urinary trypsin inhibitor is in the biological sample.
A diagnostic kit for determining the presence of urinary trypsin inhibitor in a biological fluid, the kit comprising:
- (a) trypsin;
- and
- (b) a trypsin substrate of the formula (I)
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- wherein R1 is a protecting group for Nα;
- R2 is a protecting group for NG;
- and
- R3 is aryl;
- and
- wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O—C single bond, which liberates R3—OH.
Dependencies are inferred from the claim text.