Magnetic particles for purifying nucleic acids
AssigneeRoche Molecular Systems, Inc.
InventorsHerbert Harttig, Michael Riedling, Martin Mennig, Helmut Schmidt
Life of this patent
Estimating the term…
Abstract
Preparations of particles having a glass surface, wherein more than 75% by weight of these particles have a particle size between 0.5 μm and 15 μm and a glass surface which contains between 2 and 6 mole % zinc oxide, have proven to be particularly advantageous in processes for the purification of nucleic acids. This in particular results in an increased nucleic acid yield.
Claims15
Preparation containing particles having a glass surface, wherein more than 75% by weight of these particles have a particle size between 0.5 and 15 μm.
Preparation as claimed in one of the previous claims, wherein the particles have a glass coat which contains between 2 and 6 mole % zinc oxide.
Preparation as claimed in one of the previous claims, containing at least one core of a magnetic metal.
Process for producing a preparation of particles having a core coated with a gel layer containing less than 5% by weight particles without cores comprising the steps
- suspending core particles in a sol using a core particle preparation
- spray drying the suspension to form a gel,
- wherein the core particle preparation comprises 75% by weight particles having a particle size between 0.5 and 15 μm.
Process for purifying nucleic acids by non-covalently binding nucleic acids from a sample to particles having a glass surface, removing non-bound sample components and eluting the bound nucleic acids from the glass surface, wherein the sample is contacted with a preparation as claimed in one of the claims 1 to 8 or 15 to 16.
Use of zinc oxide in glass layers generated by a sol/gel process to increase the binding capacity of the glass surface for nucleic acids.
Process for producing a preparation of particles having a core coated with a gel layer containing less than 5% by weight particles without cores comprising the steps
- suspending core particles in a sol using a core particle preparation
- spray drying the suspension to form a gel,
- compressing the gel to form a glass,
- wherein the core particle preparation comprises 75% by weight particles having a particle size between 0.5 and 15 μm.
Dependencies are inferred from the claim text.