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US 2003/0125529 A1Published application
Composite chromatographic sorbent of mineral oxide beads with hydroxyapatite-filled pores
AssigneeCiphergen Biosystems, Inc.
InventorsEgisto Boschetti, Pierre Girot
Life of this patent
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Abstract
A new adsorbent of a porous mineral oxide material with apatite crystals, preferably hydroxyapatite crystals, in the pores of the mineral oxide material is disclosed. The adsorbent is useful for protein and nucleic acid separations
Claims41
A composite chromatography sorbent comprising:
- porous mineral oxide beads that have a pore volume which exceeds about 10% of the bead volume and an average pore diameter of at least about 500 Å,
- wherein the pores of the beads contain apatite crystals.
A chromatography column, comprising:
- (a) a tubular member having an inlet end and an outlet end;
- (b) first and second porous members disposed within said tubular member;
- and
- (c) a composite chromatography sorbent comprising:
- porous mineral oxide beads that have a pore volume which exceeds about 10% of the bead volume and an average pore diameter of at least about 500 Å, wherein the pores of the beads contain apatite crystals, and wherein said composite chromatography is packed within said tubular member between said first and second porous members.
2 more elements
A chromatographic separation method comprising:
- contacting a solution comprising biomolecules with a composite chromatography sorbent,
- wherein said composite chromatography sorbent comprises porous mineral oxide beads that have a pore volume which exceeds about 10% of the bead volume and an average pore diameter of at least about 500 Å, wherein the pores of the beads contain apatite crystals, wherein the solution permeates the pores of the mineral oxide beads, and wherein some biomolecules in the solution are bound to the apatite crystals and other, different biomolecules remain in the solution.
A chromatographic separation method comprising:
- a first flowing solution comprising biomolecules through a chromatography column comprising:
- (a) a tubular member having an inlet end and an outlet end;
- (b) first and second porous members disposed within said tubular member;
- and
- (c) a composite chromatography sorbent comprising porous mineral oxide beads that have a pore volume which exceeds about 10% of the bead volume and an average pore diameter of at least about 500 Å, wherein the pores of the beads contain apatite crystals, and wherein said composite chromatography is packed within said tubular member between said first and second porous members,
- wherein the solution permeates the pores of the mineral oxide beads, and wherein some biomolecules in the solution are bound to the apatite crystals and other, different biomolecules remain in the solution.
3 more elements
A chromatographic separation method comprising:
- a first flowing solution comprising biomolecules through a chromatography column comprising:
- (a) a tubular member having an inlet end and an outlet end;
- (b) first and second porous members disposed within said tubular member;
- (c) a composite chromatography sorbent comprising porous mineral oxide beads that have a pore volume which exceeds about 10% of the bead volume and an average pore diameter of at least about 500 Å, wherein the pores of the beads contain apatite crystals, and wherein said composite chromatography is packed within said tubular member between said first and second porous members;
- and
- (d) means for flowing a liquid sample upward through said composite chromatography sorbent,
- wherein the solution permeates the pores of the mineral oxide beads, and wherein some biomolecules in the solution are bound to the apatite crystals and other, different biomolecules remain in the solution.
4 more elements
Dependencies are inferred from the claim text.