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US 2003/0125527 A1Published application
Method for single-step attachment of a label to target molecules
AssigneeMiRus LLC
InventorsJon A. Wolff, James E. Hagstrom, Paul Michael Slattum, Vladimir Budker
Life of this patent
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Abstract
Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
Claims11
A labeling molecule comprising:
- a covalently attachable labeling reagent for alkylating a chemical in a single-step reaction thereby providing the chemical with a detectable reporter molecule.
A kit comprising:
- a) a receptacle containing a covalently attachable labeling reagent for alkylating a chemical in a single-step reaction;
- and,
- b) instructions for use.
A compound having the structure comprising:
- D—B—A
- wherein,
- D is selected from the group consisting of fluorescence-emitting compounds, radioactive compounds, haptens, immunogenic molecules, chemiluminescence-emitting compounds, and proteins;
- B is selected from the group of molecules having an affinity for nucleic acids by interactions consisting of electrostatic, minor groove binding, major groove binding, and intercalation;
- and,
- A is selected from the group of alkylating agents consisting of mustards and three-membered ring derivatives.
3 more elements
A compound having the structure comprising:
- 3
- wherein,
- D is selected from the group consisting of fluorescence-emitting compounds, radioactive compounds, haptens, immunogenic molecules, chemiluminescence-emitting compounds, and proteins;
- R is selected from the group of alkyls and hydrogen;
- R″ is selected from the group of alkyls and hydrogen;
- n is an integer from 1 to 20;
- m is an integer from 1 to 20;
- x is an integer from 1 to 5;
- and,
- A is selected from the group of alkylating agents consisting of mustards and three-membered ring derivatives.
7 more elements
Dependencies are inferred from the claim text.