Methods and compositions for peptide synthesis
AssigneeTrimeris, Inc.
InventorsBrian Bray, Marc Andersen, Paul Erickson Friedrich, Myung-Chol Kang
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Abstract
The present invention relates, first, to methods for the synthesis of peptides referred to as T-1249 and T-1249-like peptides. Such methods utilize solid and liquid phase synthesis procedures to synthesize and combine groups of specific peptide fragments to yield the peptide of interest. The present invention further relates to individual peptide fragments which act as intermediates in the synthesis of the peptides on interest (e.g., T-1249). The present invention still further relates to groups of such peptide intermediate fragments which can be utilized together to produce full-length T-1249 and T-1249-like peptides.
Claims34
A method for synthesizing a peptide of the formula:
- X-WQEWEQKITALLEQAQIQQEKNEYELQKLDKWASLWEWF-Z (SEQ ID NO:
- 1), comprising:
- (a) reacting a side-chain protected peptide of the formula:
- EQAQIQQEKNEYELQKLDKWASLWEWF-Z (SEQ ID NO:
- 6), wherein the amino terminus is deprotected;
- with a side-chain protected peptide of the formula:
- X-WQEWEQKITALL-COOH (SEQ ID NO:
- 2), to yield a side-chain protected peptide of the formula:
- X-WQEWEQKITALLEQAQIQQEKNEYELQKLDKWASLWEWF-Z (SEQ ID NO:
- 1);
- wherein X is a protecting group, an acetyl group or a macromolecular carrier group;
- and wherein Z is a protecting group, an amido group, or a macromolecular carrier group.
A set of peptide fragments comprising a set selected from the group consisting of:
- 7 (a) WQEWEQKITALL, (SEQ ID NO:2) EQAQIQQEKNEYEL, (SEQ ID NO:3) QKLDKWASLWEW;
- (SEQ ID NO:4) (b) WQEWEQKITALL, (SEQ ID NO:2) EQAQIQQEKNEYEL, (SEQ ID NO;3) QKLDKWASLWEWF;
- (SEQ ID NO:5) (c) WQEWEQKITALL, (SEQ ID NO:2) EQAQIQQEKNEYELQKLDKWASLWEWF;
- (SEQ ID NO:6) (d) WQEWEQKITALL, (SEQ ID NO:2) EQAQIQQEKNEYELQKLDKWASLWEW;
- (SEQ ID NO:8) (e) WQEWEQKITALLEQAQIIQQEKNEYEL, (SEQ ID NO:7) QKLDKWASLWEW;
- and (SEQ ID NO:4) (f) WQEWEQKITALLEQAQIIQQEKNEYEL, (SEQ ID NO:7) QKLDKWASLWEWF. (SEQ ID NO:5)
A peptide selected from the group consisting of:
- 14 WQEWEQKITALL, (SEQ ID NO:2) EQAQIQQEKNEYEL, (SEQ ID NO:3) QKLDKWASLWEW, 9SEQ ID NO:4) QKLDKWASLWEWF, (SEQ ID NO:5) EQAQIQQEKNEYELQKLDKWASLWEWF, (SEQ ID NO:6) WQEWEQKITALLEQAQIIQQEKNEYEL, and (SEQ ID NO:7) EQAQIQQEKNEYELQKLDKWASLWEW. (SEQ ID NO:8)
Dependencies are inferred from the claim text.