Modified carbon & germanium surfaces
AssigneeWisconsin Alumni Research Foundation
InventorsTodd C. Strother, Lloyd M. Smith, Robert J. Hamers
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Abstract
Chemically-modified surfaces on unoxidized carbon, silicon, and germanium substrates are disclosed. Ultraviolet radiation mediates the reaction of protected ω-modified, α-unsaturated aminoalkenes (preferred) with hydrogen-terminated carbon, silicon, or germanium surfaces. Removal of the protecting group yields an aminoalkane-modified silicon surface These amino groups can be coupled to terminal-modified oligonucleotides using a bifunctional crosslinker, thereby permitting the preparation of modified surfaces and arrays. Methods for controlling the surface density of molecules attached to the substrate are also disclosed.
Claims30
A method of creating modified carbon, silicon, and germanium surfaces comprising:
- (a) reacting a straight, branched, or cyclic alkene having a substitutent thereon with an unoxidized carbon, silicon, or germanium substrate to yield a modified substrate having bonded directly thereto, in the absence of any intervening oxygen atoms, substituted alkyl moieties.
A method of creating modified surfaces on unoxidized carbon, silicon, and germanium surfaces comprising:
- (a) reacting an amino-, carboxy, or thiol-modified and protected alkene with an unoxidized carbon, silicon, or germanium substrate to yield a surface of protected, amino-, carboxy, or thiol-modified alkane molecules covalently bonded to the substrate in the absence of any intervening oxygen molecules;
- then
- (b) deprotecting the modified alkane molecules of step (a), thereby yielding a surface of unprotected modified alkane molecules bonded to the substrate;
- then
- (c) reacting the deprotected modified alkane molecules of step (b) with a crosslinker, whereby the crosslinker is attached to the modified alkane molecules;
- and then
- (d) attaching molecules to the crosslinker of step (c), thereby yielding a surface of molecules immobilized on the substrate.
A modified carbon, silicon or germanium substrate, the modified substrate comprising:
- a substrate selected from the group consisting of unoxidized, hydrogen-terminated carbon, silicon, and germanium;
- a layer of substituted alkane molecules bonded directly to the substrate, in the absence of any intervening oxygen molecules, the substituted alkane molecules having attached thereto a substituent selected from the group consisting of an amino-containing substituent, a carboxy-containing substituent, and a thiol-containing substituent.
Dependencies are inferred from the claim text.