Agents that modulate DNA-PK activity and methods of use thereof
OwnerEyal Raz, Augusto Lois +1inventors
InventorsEyal Raz, Augusto Lois, Kenji Takabayashi
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for modulating cell death in a eukaryotic cell, and methods for reducing DNA damage in a eukaryotic cell. The methods generally comprise modulating a biological activity of DNA-PK in a cell. The invention further provides methods of treating a condition related to cell death in an individual. The invention further provides methods of identifying agents which modulate a biological activity of DNA-PK, as well as agents identified by the methods. Methods of modulating an immune response using an identified agent are also provided.
Claims20
A method of modulating cell death in a eukaryotic cell, comprising contacting the cell with an agent that modulates a biological activity of DNA-PK.
A method of reducing cell damage mediated by a hypoxic condition, comprising contacting the cell with an agent that modulates a biological activity of DNA-PK.
A method for identifying an agent that modulates a biological activity of DNA-PK, comprising:
- a) adding an agent to be tested to a sample, the sample comprising DNA-PK and an immunomodulatory nucleic acid molecule, under conditions which favor binding of the immunomodulatory nucleic acid molecule to DNA-PK, thereby forming a test sample;
- and
- b) detecting a biological activity of DNA-PK protein in the test sample, as compared to a control sample lacking the agent, wherein an increase or a decrease in the biological activity of DNA-PK indicates that the agent modulates a biological activity of DNA-PK.
A method for reducing DNA damage in a eukaryotic cell, comprising contacting the cell with an agent that modulates a biological activity of DNA-PK.
A method of reducing cell death in an individual, comprising administering to an individual an effective amount of an agent that modulates a biological activity of DNA-PK.
A method of reducing cell death in an organ, comprising contacting a cell of the organ with an effective amount of an agent that modulates a biological activity of DNA-PK.
Dependencies are inferred from the claim text.