Covalently closed nucleic acid molecules for immunostimulation
AssigneeMologen Forschungs-, Entwicklungs- und Vertriebs GmbH
InventorsClaas Junghans, Burghardt Wittig, Sven Konig Merediz, Matthias Schroff
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Abstract
Short deoxyribonucleic acid molecules that are partially single-stranded, dumbbell-shaped, and covalently closed, which contain one or more unmethylated cytosine guanosine motif (CpG motif) and exhibit immunomodifying effects. Such molecules can be used for immunostimulation applications in humans or vertebrates.
Claims42
A partially single-stranded, dumbbell-shaped, covalently closed deoxyribonucleic acid molecule, containing one or more sequences having the formula:
- N1N2CGN3N4
- wherein:
- N1N2 is selected from the group consisting of GT, GG, GA, AT, and AA;
- and
- N3N4 is selected from the group consisting of CT, TT, C deoxycytosine, G deoxyguanosine, A deoxyadenosine, and T deoxythymidine.
A partially single-stranded, dumbbell-shaped, covalently closed deoxyribonucleic acid molecule, comprising at least one base sequence AACGTTCTTC GGGGCGTT as in SEQ ID NO:
- 1.
A partially single-stranded, dumbbell-shaped, covalently closed deoxyribonucleic acid molecule, comprising at least one sequence selected from the group consisting of:
- (a) SEQ ID NO:
- 2;
- (b) SEQ ID NO:
- 3;
- and
- (c) SEQ ID NO:
- 1.
A deoxyribonucleic acid molecule, consisting of a partially single-stranded, dumbbell-shaped, covalently closed chain of deoxyribonucleoside residues, and containing one or more sequences of the base sequence N1N2CGN3N4, whereby N1N2 is an element of the GT, GG, GA, AT or AA group, N3N4 is an element of the CT or TT group, as well as C deoxycytosine, G deoxyguanosine, A deoxyadenosine and T deoxythymidine, characterized by its sequence being
- a) GTTCCTGGAG ACGTTCTTAG GAACGTTCTC CTTGACGTTG GAGAGAAC or
- b) ACCTTCCTTG TACTAACGTT GCCTCAAGGA AGGTTGATCT TCATAACGTT GCCTAGATCA, or
- c) containing a deoxyribonucleic acid sequence of the base sequence AACG TTCTTCGGGG CGTT,
- d) and whereby the deoxyribonucleic acid molecule has a length of 40 to 200 nucleotides.
Use of deoxyribonucleic acid molecules consisting of a partially single-stranded, dumbbell-shaped, covalently closed chain of deoxyribonucleoside residues, and containing one or more sequences of the base sequence N1N2CGN3N4, whereby N1N2 is an element of the GT, GG, GA, AT or AA group, N3N4 is an element of the CT or TT group, as well as C deoxycytosine, G deoxyguanosine, A deoxyadenosine and T deoxythymidine, for immunostimulation applications in humans or higher animals.
A deoxyribonucleic acid molecule, consisting of a partially single-stranded, dumbbell-shaped, covalently closed chain of deoxyribonucleoside residues, and containing one or more sequences of the base sequence N1N2CGN3N4, whereby N1N2 is an element of the GT, GG, GA, AT or AA group, N3N4 is an element of the CT or TT group, as well as C deoxycytosine, G deoxyguanosine, A deoxyadenosine and T deoxythymidine, characterized by its sequence being
- 12 a) GTTCCTGGAG ACGTTCTTAG GAACGTTCTC CTTGACGTTG GAGAGAAC or b) ACCTTCCTTG TACTAACGTT GCCTCAAGGA AGGTTGATCT TCATAACGTT GCCTAGATCA, or c) containing a deoxyribonucleic acid sequence of the base sequence AACG TTCTTCGGGG CGTT.
Dependencies are inferred from the claim text.