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AssigneeWisconsin Alumni Research Foundation
InventorsLaura L. Kiessling, Laura E. Strong, Jason E. Gestwicki
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Abstract of Disclosure This invention provides multivalent ligands which carry or display at least one recognition element (RE), and preferably a plurality of recognition elements, for binding directly or indirectly to cells or other biological particles or more generally by binding to any biological molecule. The multivalent ligands provided can most generally function for binding or targeting to any biological particle or molecule and particularly to targeting of cells or cell types or viruses, for cell aggregation and generally for macromolecular assembly of biological macromolecules. The multivalent ligands of this invention are generally applicable for creating scaffolds (assemblies) of chemical or biological species, including without limitation, antigens, epitopes, ligand binding groups, ligands for cell receptors (cell surface receptors, transmembrane receptors and cytoplasmic receptors), and various macromolecules (nucleic acids, carbohydrates, saccharides, proteins, peptides, etc.). In these scaffolds, the number, spacing, relative positioning and relative orientation of recognition elements can be controlled. Multivalent ligands of this invention can carry or display at least one signal recognition element (SRE), and preferably a plurality of signal recognition elements, and modulate biological responses in biological systems. The invention also relates to methods for aggregating biological particles and macromolecules and for modulating biological response employing the multivalent ligands provided.
A method for inducing a biological response in a biological system comprising one or more receptors which comprises the step of introducing into the biological system a multivalent ligand which comprises a plurality of signal recognition elements recognized by at least one of the receptors and bonded to a molecular scaffold.
A method for treating a bacterial infection which comprises the step of administering a therapeutically effective amount of a multivalent ligand to an individual having a bacterial infection wherein the multivalent ligand comprises a plurality of signal recognition elements that are chemoattractant signals covalently bonded to a molecular scaffold.
A pharmaceutical composition for treating a bacterial infection which comprises an amount of a multivalent ligand effective for inhibiting the chemotaxis response in the bacterium, wherein the multivalent ligand comprises a plurality of signal recognition elements that are chemoattractant signals covalently bonded to a molecular scaffold, and a pharmaceutically acceptable carrier.
A method for modulating the chemotaxis response of a eukaryotic cell which comprises the step of contacting the eukaryotic cell with a multivalent ligand which comprises a plurality of signal recognition elements that are chemoattractants of the eukaryotic cell.
A method for treating an infection of a eukaryotic pathogen or parasite which comprises the step of administering a therapeutically effective amount of a multivalent ligand to an individual having an infection wherein the multivalent ligand comprises a plurality of signal recognition elements that are chemoattractants of the pathogen or parasite covalently bonded to a molecular scaffold.
A pharmaceutical composition for treating an infection of a eukaryotic pathogen or parasite which comprises an amount of a multivalent ligand effective for inhibiting the chemotaxis response in the pathogen or parasite, the multivalent ligand comprising a plurality of signal recognition elements that are chemoattractants covalently bonded to a molecular scaffold, and a pharmaceutically acceptable carrier.
A multivalent ligand having the structure:
A method for enhancing aggregation of biological particles which comprises the steps of:
A method for inducing or enhancing induction of apoptosis in a cell which comprises the steps of:
A method for generating an assembly of biological macromolecules or particles which comprises the steps of:
Dependencies are inferred from the claim text.