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US 2003/0125208 A1Published application
Use of tobacco mild green mosaic virus (tmgmv) mediated lethal hypersensitive response (hr) as a novel method of weed control
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsRaghavan Charudattan, Matthew Scott Pettersen, Ernest Hiebert
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Abstract
Tobacco mild green mosaic virus (TMGMV) induces a lethal, systemic, hypersensitive response in Tropical Soda Apple (TSA). TMGMV is a member of the tobamoviruses, which consist of mechanically transmitted, rod-shaped, RNA viruses that are strictly plant pathogens.
Claims14
A method of inducing lethal hypersensitive response in tropical soda apple plants comprising the steps of:
- (a) obtaining an inoculation solution comprising Tobacco Mild Green Mosaic Virus, buffer and water;
- (b) applying said inoculation solution to the adaxial surface of the leaves of said tropical soda plant by sprayer application.
A method of inducing lethal hypersensitive response in tropical soda apple plants comprising the steps of:
- (a) extracting Tobacco Mild Green Mosaic Virus from host plant tissue, by triturating host plant tissue in a buffer, wherein the ratio of host plant tissue to buffer is up to 1 g/ml;
- (b) filtering extracted virus sample through a filter;
- (c) preparing an inoculation solution by diluting the extracted sample with water;
- (d) applying said inoculation solution to the adaxial surface of the leaves of said tropical soda apple plant.
1 more element
Dependencies are inferred from the claim text.