Method of production of purified hepatitis a virus particles and vaccine preparation
AssigneeBaxter Healthcare S.A.
InventorsChrista Tauer, Heidi Meyer, Artur Mitterer, Noel Barrett
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Abstract
The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune response in a mammal.
Claims51
A method of producing complete Hepatitis A Virus particles comprising the steps of treating an HAV preparation from a cell culture supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, and isolating said purified preparation of complete HAV particles.
A method of production of a purified Hepatitis A Virus preparation comprising the steps of treating the HAV preparation from the supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, isolating said purified preparation of complete HAV particles and isolating purified mature HAV virions from said preparation complete HAV particle.
A method of production of a purified complete HAV particle preparation consisting which consists of purifying complete HAV particles from a cell culture supernatant an HAV infected cells by filtering.
A method of production of a purified mature HAV particle preparation which consists of purifying mature HAV particles from a cell culture supernatant of HAV infected cells by filtering and centrifugation.
A method of isolating complete HAV particle from a cell-free cell culture supernatant of HAV infected cells comprising the steps of treating the HAV preparation from the supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, and isolating said purified preparation of complete HAV particles.
A method of isolating mature HAV particle from a cell culture supernatant HAV harvest of HAV infected cells comprising the steps of treating the HAV preparation from the supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, isolating said purified preparation of complete HAV, and isolating from said preparation of complete HAV particle a preparation consisting of mature HAV particle.
A preparation of complete HAV particle free of HAV precursor polypeptide and contaminating cell or cell culture protein.
A preparation of purified mature HAV particle free of HAV precursor polypeptide and contaminating cell or cell culture protein.
A method for production of HAV vaccine comprising the steps of treating an HAV preparation from the cell culture supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, and isolating said purified preparation of complete HAV particles, and preparing an immunogenic composition comprising a preparation consisting of purified, complete HAV virions.
A method for the production of an HAV vaccine comprising the steps of treating the HAV preparation from the supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, isolating said purified preparation of complete HAV particles and isolating purified mature HAV virions from said preparation complete HAV particle, and preparing an immunogenic composition comprising a preparation consisting of purified, mature HAV virions.
A method for production of an inactivated HAV vaccine comprising the steps of treating the HAV preparation from the supernatant of an HAV infected cell culture with a nucleic acid degrading agent and a protease, isolating said purified preparation of complete HAV particles and isolating purified mature HAV virions from said preparation complete HAV particle, and preparing an immunogenic composition comprising a preparation, wherein the HAV preparation is treated with an inactivation agent prior or after isolating of mature HAV virion particle.
Dependencies are inferred from the claim text.