Vector encoding suicide and marker constructs
OwnerPaul Orchard, Victor Lewisinventors
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Abstract
The present invention provides a vector encoding a detectable cell surface marker and a suicide construct, and cells and a non-human mammal transduced with this vector. Introduction of lymphocytes transduced with this vector, after allogeneic bone marrow transplantation, serves to treat or prevent complications from the bone marrow transplant, including graft versus host disease.
Claims32
A chimeric vector, comprising a first nucleic acid region encoding an extracellular domain of a protein, and a second nucleic acid region encoding a cytosine deaminase (CD), operably linked to the first region.
A T lymphocyte comprising a first nucleic acid segment encoding the transmembrane and extracellular domains of the human nerve growth factor receptor, a second nucleic acid segment encoding a Saccharomyces cerevisiae cytosine deaminase, and third nucleic acid segment encoding a (gly4ser)2 linker, operably linked to the first and second nucleic acid segments.
A nucleic acid sequence comprising a first nucleic acid segment encoding the transmembrane and extracellular domains of the human nerve growth factor receptor, a second nucleic acid segment encoding a Saccharomyces cerevisiae cytosine deaminase, and third nucleic acid segment encoding a (gly4ser)2 linker, operably linked to the first and second nucleic acid segments.
Dependencies are inferred from the claim text.