Compositions and methods of use of mammalian retrotransposons
AssigneeThe Trustees of the University of Pennsylvania
InventorsHaig H. Kazazian JR., Eric Ostertag, Ralph DeBerardinis
Life of this patent
Estimating the term…
Abstract
The invention relates to an isolated DNAc molecule comprising a promoter P and an L1 cassette sequence comprising a core L1 retrotransposon element and methods of use thereof.
Claims22
A method for generating a mutation in an offspring of an animal, the method comprising creating an insertional mutation in a genome of an animal comprising breeding a first animal with a second animal, wherein the first animal is a transgenic animal comprising an isolated DNAc molecule, thereby generating a mutation in the offspring of the animal.
A method of isolating a nucleic acid molecule from a genome of an offspring of an animal, the method comprising creating an insertional mutation in a genome of an animal comprising breeding a first animal with a second animal, wherein the first animal is a transgenic animal comprising an isolated DNAc molecule, the method further comprising detecting the DNAc molecule and a nucleic acid molecule flanking the insertion site of the isolated DNAc molecule, thereby isolating the nucleic acid molecule from the genome of the offspring of the animal.
A method of creating a transgenic offspring of an animal, the method comprising breeding a first animal with a second animal, wherein the first animal is a transgenic animal comprising an isolated DNAc molecule, thereby creating a transgenic offspring of an animal.
A method for creating an insertional mutation in the germ line of an animal, the method comprising introducing a nucleic acid molecule into an animal, wherein the nucleic acid molecule comprises a germ line specific promoter, thereby creating an insertional mutation in the germ line of an animal.
Dependencies are inferred from the claim text.