Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix

A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:

• providing a solid-phase extraction material comprising hydrophilic particles disposed on a layer of a hydrophobic matrix and at least partially embedded therein;
• providing a biological sample mixture;
• and
• contacting the biological sample mixture with the solid-phase extraction material to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.

A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:

• providing a solid-phase extraction material comprising a hydrophilic solid support at least partially embedded within a hydrophobic matrix;
• providing a biological sample mixture;
• and
• contacting the biological sample mixture with the solid-phase extraction material to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture;
• wherein the biological sample mixture comprises a nucleic acid amplification reaction mixture.

A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:

• providing a device comprising at least one process array that comprises a solid-phase extraction material, wherein the solid-phase extraction material comprises a hydrophilic solid support at least partially embedded within a hydrophobic matrix;
• providing a biological sample mixture in the at least one process array;
• and
• transferring the biological sample mixture within the at least one process array, wherein the biological sample mixture and the solid-phase extraction material remain in contact for a sufficient time to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.

A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:

• providing a device comprising at least one process array that comprises a solid-phase extraction material, wherein the solid-phase extraction material comprises hydrophilic particles disposed on a layer of a hydrophobic matrix and at least partially embedded therein;
• providing a biological sample mixture in the at least one process array;
• and
• transferring the biological sample mixture within the at least one process array, wherein the biological sample mixture and the solid-phase extraction material remain in contact for a sufficient time to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.

A device for use in removing small negatively charged organic molecules from a biological sample mixture, the device comprising:

• a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
• a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
• at least one distribution channel connecting the plurality of process chambers;
• and
• a solid-phase extraction material within at least one of the process arrays, wherein the solid-phase extraction material comprises a hydrophilic solid support at least partially embedded within a hydrophobic matrix.