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AssigneeCold Spring Harbor Laboratory
InventorsGrigori N. Enikolopov, Natalia I. Peunova, Boris A. Kuzin, Hollis Cline, Tatiyana Michurina
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The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells in bone marrow which are capable of undergoing normal hematopoiesis and differentiation, wherein the bone marrow is contacted with an inhibitor of NO, such as an inhibitor of nitric oxide synthase (NOS), thereby producing bone marrow having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g., blood, skin, bone and digestive epithelium), or precursor cells of the selected tissue, with an inhibitor of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue.
A method of increasing in a mammal a population of hematopoietic stem cells in bone marrow which are capable of undergoing normal hematopoiesis and differentiation, comprising contacting the bone marrow with an inhibitor of nitric oxide synthase, thereby producing bone marrow having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation.
A method for treating a mammal to increase a population of hematopoietic stem cells in bone marrow of the mammal which are capable of undergoing normal hematopoiesis and differentiation, comprising contacting the bone marrow of the mammal with an inhibitor of nitric oxide synthase, thereby producing bone marrow having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation.
A method for treating a mammal to increase a population of hematopoietic stem cells in bone marrow of the mammal which are capable of undergoing normal hematopoiesis and differentiation, comprising the steps of:
A method of increasing a population of dividing cells in a tissue of a mammal comprising contacting the cells with an inhibitor of nitric oxide.
A method of decreasing a population of cells in S phase in a tissue of a mammal and inducing differentiation of the cells, comprising contacting the tissue with an enhancer of nitric oxide.
A method of coordinating developmental decisions of a cell type in a mammal, comprising introducing nitric oxide into the cell type or precursor of the cell type, thereby inhibiting proliferation of the cell type or precursor of the cell type and inducing differentiation of the cell type or precursor of the cell type.
A method of inducing differentiation in a mammalian cell population comprising contacting the cell population with nitric oxide or a nitric oxide enhancer.
A method of regenerating tissue in an adult mammal comprising contacting a selected tissue with an inhibitor of nitric oxide, thereby inhibiting differentiation and inducing proliferation of cells of the tissue, then contacting the selected tissue with a compound which inhibits proliferation and induces differentiation.
A method of repopulating an organ or tissue having normally nondividing cells comprising contacting a selected organ or tissue with an inhibitor of nitric oxide, thereby inhibiting differentiation and inducing proliferation of cells of the organ or tissue, then contacting the selected organ or tissue with a compound which inhibits proliferation and induces differentiation.
A method of producing a subpopulation of hematopoietic cells comprising the steps of:
A method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor of nitric oxide.
Dependencies are inferred from the claim text.