US 2003/0119771 A1 · Modulators of bone homeostasis identified in a high-throughput screen

Abstract

The invention relates to the field of molecular genetics and medicine. In particular, the present invention relates to the field of functional genomics, i.e., to a method for the identification of genes that function in regulating bone homeostasis, such as the induction of osteogenesis. In particular, the present invention relates to polynucleotides and the encoded polypeptides that are identified in a high-throughput screen designed to detect modulation of bone alkaline phosphatase activity. Moreover, the present invention relates to vectors, host cells, antibodies and diagnostic methods for detecting diseases involving the discovered polynucleotides, and therapeutic methods for treating such diseases. The invention further relates to methods and means for drug compound screens designed to develop new therapeutic strategies.

Claims39

Dependent claims

An isolated nucleic acid selected from a group of nucleic acids identifiable as inducers of osteoblast differentiation consisting of:

(a) a DNA sequence of SEQ ID NO:
1, 3, 5, 7, or 9 or the complement thereof,
(b) a RNA sequence corresponding to SEQ ID NO:
1, 3, 5, 7, or 9 or the complement thereof,
(c) a nucleic acid specifically hybridizing to one of the nucleotide sequences defined in (a) or (b),
(d) a nucleic acid encoding a protein comprising the amino acid sequence of SEQ ID NO:
2, 4, 6, 8, or 10,
(e) a nucleic acid encoding an immunologically active and/or functional fragment of a protein encoded by a DNA sequence of SEQ ID NO:
1, 3, 5, 7, or 9,
(f) a nucleic acid encoding a protein as defined in SEQ ID NO:
2, 4, 6, 8, or 10, or a nucleic acid as defined in any one of (a) to (e) wherein said sequence is bNA, cDNA, genomic DNA or synthetic DNA.

8 more elements

Dependencies are inferred from the claim text.

Modulators of bone homeostasis identified in a high-throughput screen

Life of this patent

Modulators of bone homeostasis identified in a high-throughput screen

Life of this patent

Abstract

Claims39