Loading patent…
AssigneeThe Trustees of the University of Pennsylvania
InventorsGuangping Gao, James M. Wilson
Estimating the term…
A method for producing recombinant adeno-associated virus in the absence of contaminating helper virus or wild-type virus involves culturing a mammalian host cell containing an rAd/AAV hybrid virus, an AAV rep sequence and an AAV cap sequence under the control of regulatory sequences directing expression thereof. The rAd/AAV hybrid virus contains a rAAV construct to be packaged into an AAV virion in an backbone containing the adenoviral sequences necessary to express E1a and E1b gene products and to permit replication of the hybrid virus. The method of the invention permits replication of the hybrid virus and production of rAAV virion in this host cell in the absence of a helper virus and obviates a subsequent purification step to purify rAAV from contaminating virus.
An adenovirus/AAV hybrid virus comprising a recombinant adeno-associated viral (rAAV) vector, and nucleic acid sequences encoding adenovirus E1 a and adenovirus E1b are under the control of regulatory sequences directing expression of the E1a and E1b gene products, wherein said hybrid virus contains sufficient adenoviral sequences to permit replication of said hybrid virus in a selected host cell and wherein said rAAV comprises sequences selected from AAV type 1 or AAV type 5.
An adenovirus/AAV hybrid virus comprising:
14 The hybrid adenovirus/AAV virus according to claim 13, wherein said hybrid adenovirus/AAV virus further comprises nucleic acid sequences encoding adenoviral E2a gene product under the control of regulatory sequences which direct expression thereof in a host cell.
Dependencies are inferred from the claim text.