Plant transcription factors
OwnerRebecca E. Cahoon, Guihua Lu +1inventors
InventorsRebecca E. Cahoon, Guihua Lu, Mark E. Williams
Life of this patent
Estimating the term…
Abstract
This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the transcription factor in a transformed host cell.
Claims77
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 68 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6 and 8, or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 68 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group, consisting of SEQ ID NOs:2, 4, 6 and 8.
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 160 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of FUSCA transcription factor polypeptides of SEQ ID NOs:10 and 12, or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 160 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:10 and 12.
An isolated polynucleotide comprising a first nucleotide sequence encoding a first polypeptide of at least 190 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:18 and 20, or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 190 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:18 and 20.
An isolated polynucleotide comprising a first nucleotide sequence encoding a first polypeptide of at least 95 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to SEQ ID NO:14, or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 95 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:14.
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 190 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:24, 26, 32 and 38 or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 190 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO:24, 26, 32 and 38.
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 80 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to SEQ ID NO:34 or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 80 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:34.
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 300 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:42 and 44, or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
A polypeptide of at least 300 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:42 and 44.
A method of selecting an isolated polynucleotide that affects the level of expression of a transcription factor polypeptide in a plant cell, the method comprising the steps of:
- (a) constructing an isolated polynucleotide comprising a nucleotide sequence of at least one of 30 contiguous nucleotides derived from a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43 and the complement of such nucleotide sequences;
- (b) introducing the isolated polynucleotide into a plant cell;
- and
- (c) measuring the level of a polypeptide in the plant cell containing the polynucleotide to provide a positive selection means.
A method of obtaining a nucleic acid fragment encoding a transcription factor polypeptide comprising the steps of:
- (a) synthesizing an oligonucleotide primer comprising a nucleotide sequence of at least one of 30 contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 17, 19, 23, 25, 31, 33, 37, 41, 43 and the complement of such nucleotide sequences;
- and
- (b) amplifying a nucleic acid sequence using the oligonucleotide primer.
A method of obtaining a nucleic acid fragment encoding a transcription factor polypeptide comprising the steps of:
- (a) probing a cDNA or genomic library with an isolated polynucleotide comprising a nucleotide sequence of at least one of 30 contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 17, 19, 23, 25, 31, 33, 37, 41 and 43 and the complement of such nucleotide sequences;
- (b) identifying a DNA clone that hybridizes with the isolated polynucleotide;
- (c) isolating the identified DNA clone;
- and
- (d) sequencing the cDNA or genomic fragment that comprises the isolated DNA clone.
An isolated polynucleotide comprising a first nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 70% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:16, 28, 30, 34, 36, and 40.
A polypeptide of at least 50 amino acids that has at least 70% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:16, 28, 30, 34, 36, and 40.
Dependencies are inferred from the claim text.