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US 2003/0119164 A1Published application
Lysophospholipase
AssigneeNovozymes A/S
InventorsHiroaki Udagawa, Torben Peter Frandsen, Tom Anton Busk Nielsen, Markus Sakari Kauppinen, Soren Christensen
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Abstract
The inventors have isolated lysophospholipases from Aspergillus (A. niger and A. oryzae) having molecular masses of about 68 kDa and amino acid sequences of 600-604 amino acid residues. The novel lysophospholipases have only a limited homology to known amino acid sequences. The inventors also isolated genes encoding the novel enzymes and cloned them into E. coli strains.
Claims12
A lysophospholipase which is:
- a) a polypeptide encoded by a lysophospholipase encoding part of the DNA sequence cloned into a plasmid present in Escherichia coli deposit number DSM 13003, DSM 13004, DSM 13082 or DSM 13083, or
- b) a polypeptide having an amino acid sequence as the mature peptide shown in SEQ ID NO:
- 2, 4, 6 or 8, or which can be derived therefrom by substitution, deletion, and/or insertion of one or more amino acids, particularly by deletion of 25-35 amino acids at the C-terminal;
- c) an analogue of the polypeptide defined in (a) or (b) which:
- i) has at least 70% homology with said polypeptide,
- ii) is immunologically reactive with an antibody raised against said polypeptide in purified form, or
- iii) is an allelic variant of said polypeptide;
- or
- d) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with a complementary strand of the nucleic acid sequence shown as nucleotides 109-1920 of SEQ ID NO:
- 1, 115-1914 of SEQ ID NO:
- 3, 70-1881 of SEQ ID NO:
- 5 or 193-2001 of SEQ ID NO:
- 7, or a subsequence thereof having at least 100 nucleotides.
10 more elements
A nucleic acid sequence which comprises:
- a) the lysophospholipase encoding part of the DNA sequence cloned into a plasmid present in Escherichia coli DSM 13003, DSM 13004, DSM 13082 or DSM 13083,
- b) the nucleic acid sequence shown as nucleotides 109-1920 of SEQ ID NO:
- 1, 115-1914 of SEQ ID NO:
- 3, 70-1881 of SEQ ID NO:
- 5 or 193-2001 of SEQ ID NO:
- 7,
- c) an analogue of the sequence defined in a) or b) which encodes a lysophospholipase and
- i) has at least 70% homology with said DNA sequence, or
- ii) hybridizes at high stringency with a complementary strand of said DNA sequence or a subsequence thereof having at least 100 nucleotides,
- iii) is an allelic variant thereof, or
- d) a complementary strand of a), b) or c).
8 more elements
Dependencies are inferred from the claim text.