Cloning and expression of microbial phytase
OwnerRobert F.M. Van Gorcom, Willem Van Hartingsveldt +4inventors
InventorsRobert F.M. Van Gorcom, Willem Van Hartingsveldt, Petrus Andreas Van Paridon, Annemarie Eveline Veenstra, Rudolf G.M. Luiten, Gerardus C.M. Selten
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Abstract
A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.
Claims31
A purified and isolated DNA sequence characterized in that said DNA sequence encodes a peptide or protein having phytase activity.
A purified and isolated DNA sequence characterized in that said sequence exhibits at least one of the following characteristics:
- a) hybridizes to an oligonucleotide probe derived from the DNA sequence as disclosed in FIG. 6;
- b) hybridizes to an oligonucleotide probe derived from the cDNA sequence as disclosed in FIG. 8.
A phytase characterized in that the phytase exhibits the following charactistics:
- a) provides a single band at 85 kDa on SDS-PAGE when expressed in an Aspergillus host;
- b) has an apparent molecular weight after deglycosylation in the range of about 48-56.5 kDa;
- c) has a specific activity of about 100 U/mg protein.
Dependencies are inferred from the claim text.