Composition and method for hot start nucleic acid amplification
AssigneeRoche Diagnostics Operations, Inc.
InventorsWaltraud Ankenbauer, Dieter Heindl, Frank Laue, Andreas Huber
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Abstract
The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
Claims9
A composition for performing a nucleic acid amplification reaction comprising
- a thermostable DNA-Polymerase
- a thermostable 3′-5′ Exonuclease
- at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase.
A kit comprising a thermostable DNA-Polymerase, a thermostable 3′-5′ Exonuclease, and at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase
A method for performing an amplification of a template nucleic acid, comprising
- a) providing a reaction mixture comprising at least one primer which is modified at its 3′ terminal residue such that said primer is not elongated by a thermostable DNA-Polymerase
- b) removing said modification without addition of further reaction mixture components by raising the temperature of said reaction mixture, and
- c) amplification of the template nucleic acid
Dependencies are inferred from the claim text.