Recombinant polypeptides
AssigneeImmunex Corporation
InventorPranhitha Reddy
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Abstract
The present invention relates, in part, to a polypeptide domain having reduced immunogenicity that is capable of inducing trimers in heterologous polypeptides, fusion polypeptides comprising the trimer inducing domain, nucleic acids encoding the polypeptides of the invention and methods of making and using the same.
Claims21
A fusion polypeptide comprising a first polypeptide consisting of amino acids x to y of the polypeptide depicted in SEQ ID NO:
- 10, wherein x is 110 to 173 and y is 179 to 270, fused to a second heterologous polypeptide, and wherein the fusion polypeptide is capable of forming a trimer.
A fusion polypeptide comprising a first polypeptide consisting of amino acids x to y of the polypeptide depicted in SEQ ID NO:
- 10, wherein x is 110 to 173 and y is 179 to 270 with at least one substitution of an amino acid, wherein the first polypeptide is at least 80% identical to the polypeptide depicted in SEQ ID NO:
- 10 and the ability to trimerize is retained, and wherein said first polypeptide is fused to a second heterologous polypeptide.
A method of constructing a recombinant expression vector comprising the steps of linking a first nucleic acid comprising nucleotides x to y of the nucleic acid depicted in SEQ ID NO:9, wherein x is 331 to 508, and y is 546 to 808, and a second nucleic acid encoding a heterologous polypeptide to the first nucleic acid into a compatible cloning site of an expression vector, followed by amplifying and isolating said recombinant expression vector.
Dependencies are inferred from the claim text.