Method of making FGF-12 and KGF-2
AssigneeHuman Genome Sciences, Inc.
InventorMichael W. Laird
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Abstract
The present invention is directed to a method of expressing KGF-2 polypeptides in an ompT-deficient prokaryotic host cell. Also provided are ompT-deficient prokaryotic host cells comprising polynucleotides encoding KGF-2. The present invention is further directed to a method of expressing FGF-12 in an ompT-deficient prokaryotic host cell. Also provided are ompT-deficient prokaryotic hot cells comprising polynucleotides encoding FGF-12.
Claims13
An ompT deficient prokaryotic host cell comprising a nucleic acid comprising a polynucleotide encoding amino acids 68 to 208 of SEQ ID NO:2.
An ompT deficient prokaryotic host cell comprising a nucleic acid comprising a polypeptide, wherein, except for 1 to 50 amino acids substitutions, deletions, or additions, said polypeptide is selected from the group consisting of:
- (a) amino acids 1 to 208 of SEQ ID NO:2;
- (b) amino acids 37 to 208 of SEQ ID NO:2;
- (c) amino acids 63 to 208 of SEQ ID NO:2;
- and
- (d) amino acids 68 to 208 of SEQ ID NO:2,
- and wherein said polynucleotide encodes a polypeptide which stimulates proliferation of keratinocytes.
An ompT deficient prokaryotic host cell comprising a polynucleotide encoding amino acids 1 to 243 of FGF-12.
Dependencies are inferred from the claim text.